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US 8,765,380 B2Granted
Bead emulsion nucleic acid amplification
Assignee454 Life Sciences Corporation
InventorsJan Berka, Yi-Ju Chen, John H. Leamon, Steve Lefkowitz, Kenton L. Lohman, Vinod B. Makhijani, Jonathan M. Rothberg, Gary J. Sarkis, Maithreyn Srinivasan, Michael P. Weiner
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Abstract
Disclosed are methods for nucleic acid amplification wherein nucleic acid templates, beads, and amplification reaction solution are emulsified and the nucleic acid templates are amplified to provide clonal copies of the nucleic acid templates attached to the beads. Also disclosed are kits and apparatuses for performing the methods of the invention.
Claims9
A method for analyzing nucleic acid sequences comprising:
- (a) delivering a plurality of molecules of a deoxyribonucleic acid into aqueous microreactors in a water-in-oil emulsion such that a plurality of aqueous microreactors comprise a single molecule of the deoxyribonucleic acid, a single bead capable of hybridizing the deoxyribonucleic acid, and reagents necessary to perform deoxyribonucleic acid amplification;
- (b) amplifying the deoxyribonucleic acid in the microreactors to form amplified copies of said deoxyribonucleic acid bound to beads in the microreactors;
- (c) determining the presence of amplified copies of said deoxyribonucleic acid bound to a bead.
Dependencies are inferred from the claim text.