Compositions and means for diagnosing microbial infections
AssigneeStatens Serum Institut
InventorNiels Frimodt-Møller
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Abstract
The present invention pertains to the need for novel, reliable, fast and inexpensive approaches to diagnosing, including detecting and characterizing microbial infections in humans and animals or methods for detecting and characterizing microbial infections in various environments, such as in a food or feed sample. The present invention provides compositions, platforms, kits and methods for diagnosing, detecting and/or characterizing a microbial infection or contamination. In particular the present invention relates to such compositions, platforms, kits and methods for diagnosing, detecting and/or characterizing a urinary tract infection.
Claims59
A platform comprising a solid support that comprises six or more separate compartments, each compartment comprising a test composition or a control composition, wherein
- i) said test composition comprises a semi solid microbial growth medium, an antibacterial antibiotic, and three or more chromogenic substrates selected from the group consisting of 5-Bromo-6-chloro-3-indolyl phosphate p-toluidine, 5-Bromo-4-chloro-3-indolyl-β-D-galactopyranoside, 5-Bromo-6-chloro-3-indolyl-β-D-galactopyranoside, 6-Chloro-3-indolyl-β-D-galactopyranoside, 5-Bromo-4-chloro-3-indolyl-β-D-glucopyranoside, and 5-Bromo-4-chloro-3-indolyl-β-D-glucuronide;
- ii) at least five of said compartments comprise test compositions, each test composition differing from the others by comprising a different antibacterial antibiotic;
- iii) at least one of said compartments comprises a control composition;
- said control composition comprises the same semi solid microbial growth medium and the same chromogenic substrates as said test composition, but does not comprise an antibacterial antibiotic as defined in i);
- and wherein said semi solid microbial growth medium comprises tryptophan.
A platform comprising a solid support that comprises at least 5 separate test compartments, each having an area of 4-9 cm2, and at least one control compartment having an area of 15-25 cm2, wherein
- i) each of said test compartments comprises a test composition comprising a semi solid microbial growth medium, an antibacterial antibiotic selected from the group consisting of aminoglycosides, ansamycins, beta-lactam antibiotics, glycopeptides, macrolides, lincosamides, polypeptides, quinolones, sulphonamides, tetracyclines, cyclic lipopeptides, glycylcyclines, oxazolidinones, diaminopyrimidines, nitrofurans, rifamycins, antibiotic peptides, amphenicols, nitroimidazoles, streptogramins and phosphomycins,
- and
- three or more chromogenic substrates selected from the group consisting of 5-Bromo-6-chloro-3-indolyl phosphate p-toluidine, 5-Bromo-4-chloro-3-indolyl-O-D-galactopyranoside, 5-Bromo-6-chloro-3-indolyl-β-D-galactopyranoside, 6-Chloro-3-indolyl-β-D-galactopyranoside, 5-Bromo-4-chloro-3-indolyl-β-D-glucopyranoside, 5-Bromo-4-chloro-3-indolyl-β-D-glucuronide;
- ii) each test composition differs from the others only by comprising a different antibacterial antibiotic;
- iii) said control compartment comprises a control composition which comprises the same semi-solid microbial growth medium and the same chromogenic substrates as said test composition, but does not comprise said antibacterial antibiotic as defined in i);
- and wherein said semi solid microbial growth medium comprises tryptophan.
Dependencies are inferred from the claim text.