IPV-DPT vaccine
AssigneesJapan Poliomyelitis Research Institute, Takeda Pharmaceutical Company Limited
InventorsShinobu Abe, Bunsichi Simizu
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Abstract
The invention provides a process for producing a combined vaccine containing an inactivated Sabin strain of poliovirus, a Bordetella pertussis protective antigen, a diphtheria toxoid and a tetanus toxoid, the process including a step of producing a high-titer Sabin strain poliovirus. The inventive process for producing a combined vaccine, including a step of culturing, in the presence of from about 4 g/L to about 6 g/L of a microcarrier, Vero cells to be inoculated with a Sabin strain of poliovirus, is useful as a process for efficiently producing a combined vaccine containing an inactivated Sabin strain of poliovirus.
Claims10
A process for producing a combined vaccine containing,
- (A) type I, type II, and type III inactivated Sabin strains of poliovirus in a weight proportion of D antigens of (2-4):(80-120):(80-120),
- (B) a Bordetella pertussis protective antigen,
- (C) a diphtheria toxoid, and
- (D) a tetanus toxoid,
- the process comprising:
- (a) culturing Vero cells in about 4 g/L to about 6 g/L of a microcarrier, wherein the culture medium is selected from the group consisting of ME medium, DME medium, RPMI 1640 medium and RPMI 199 medium, and wherein the culture medium contains from about 5 to about 70 vol % of calf serum or fetal bovine serum;
- (b) infecting the Vero cells with type I, type II and type III Sabin strains of poliovirus;
- (c) allowing the Sabin strains to proliferate;
- (d) recovering a virus fluid containing the Sabin strains;
- (e) inactivating the Sabin strains to produce the type I, type II, and type III inactivated Sabin strains of poliovirus of (A);
- and
- (f) mixing the inactivated Sabin strains of poliovirus of (A with a diphtheria-tetanus-pertussis combined vaccine comprising the Bordetella pertussis protective antigen, diphtheria toxoid, and tetanus toxoid of (B)-(D).
Dependencies are inferred from the claim text.