Hepatitis B virus compositions and methods of use
AssigneeFox Chase Cancer Center
InventorsNing Chai, John M. Taylor
Life of this patent
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Abstract
A polypeptide comprising a preS1 region of hepatitis B virus (HBV), or a fragment thereof, and/or the preS2 region of HBV or a fragment thereof, and methods of use to inhibit virus infection are disclosed. A lentivirus comprising hepatitis B virus (HBV) envelope proteins, or a fragment thereof, and/or the L envelope protein of HBV and/or the M envelope protein of HBV or a fragment thereof, and/or the S envelope protein of HBV or a fragment thereof, and methods of use of this lentivirus HBV pseudovirus as a gene therapy to target hepatocytes for the administration of therapeutic agents are also disclosed.
Claims19
A secreted recombinant polypeptide comprising a preS1 region, wherein the preS1 region consists of the first 30 contiguous amino acids of the sequence of preS1 region of the adw2 serotype of HBV (SEQ ID NO:
- 2), wherein the polypeptide is capable of inhibiting HBV and/or HDV infection of hepatocytes, further wherein the polypeptide does not contain amino acids of the sequence of the S region of HBV.
A method of in vivo inhibition of hepatocyte infection, the method comprising administering to an animal in need thereof an effective amount of an isolated nucleic acid sequence encoding a secreted fusion polypeptide comprising:
- 1) a preS1 region, wherein the preS1 region consists of the first 30 contiguous amino acids of the sequence of preS1 region of the adw2 serotype of HBV (SEQ ID NO:
- 2);
- and 2) a constant domain sequence of an immunoglobulin or a sequence of preS2 region of HBV, wherein the polypeptide is capable of inhibiting HBV and/or HDV virus infection of hepatocytes.
An isolated polynucleotide encoding a polypeptide comprising a preS1 region, wherein the preS1 region consists of the first 30 contiguous amino acids of the sequence of preS1 region of the adw2 serotype of HBV (SEQ ID NO:
- 2), wherein the polypeptide is capable of inhibiting HBV and/or HDV virus infection of hepatocytes, further wherein the polypeptide does not contain amino acids of the sequence of the S region of HBV.
A method of in vivo inhibition of hepatocyte infection, the method comprising administering to an animal in need thereof an effective amount of an isolated secreted fusion polypeptide comprising:
- 1) a preS1 region, wherein the preS1 region consists of the first 30 contiguous amino acids of the sequence of preS1 region of the adw2 serotype of HBV (SEQ ID NO:
- 2);
- and 2) a constant domain sequence of an immunoglobulin or a sequence of preS2 region of HBV, wherein the polypeptide is capable of inhibiting HBV and/or HDV virus infection of hepatocytes.
Dependencies are inferred from the claim text.