Methods for detecting and monitoring circulating cancer stem cells
AssigneeThe Johns Hopkins University
InventorsWilliam Matsui, Richard J. Jones, Carol A. Huff
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Abstract
Provided herein are compositions, methods, and kits useful for detecting whether a subject has or is likely to develop a cancer and for monitoring, staging and examining a cancer patient. Also provide herein are methods for screening compounds.
Claims8
A method for monitoring the effectiveness of a cancer treatment in a patient, wherein the cancer treatment is for leukemia, comprising the steps of:
- (a) obtaining a blood sample from the patient;
- (b) isolating a predetermined population of cells from the blood sample, wherein the isolated cells are CD34+ and CD38−, using at least one of flow cytometry, fluorescence activated cell sorting, antibody-dependent depletion, antibody-dependent solid phase capture, panning, affinity column separation, and magnetic selection;
- (c) determining whether the isolated cells are ALDH+;
- and
- (d) comparing the number of ALDH+ cells with the number of ALDH+ cells determined at an earlier time in the cancer treatment.
A method for monitoring the effectiveness of a cancer treatment in a patient, wherein the cancer treatment is for multiple myeloma, comprising the steps of:
- (a) obtaining a blood sample from the patient;
- (b) isolating a predetermined population of cells from the blood sample, wherein the isolated cells are CD138−, CD27+, CD19+ and CD20+, using at least one of flow cytometry, fluorescence activated cell sorting, antibody-dependent depletion, antibody-dependent solid phase capture, panning, affinity column separation, and magnetic selection;
- (c) determining whether the isolated cells are ALDH+;
- and
- (d) comparing the number of ALDH+ cells with the number of ALDH+ cells determined at an earlier time in the cancer treatment.
A method for monitoring the effectiveness of a cancer treatment in a patient, wherein the cancer treatment is for Non-Hodgkin's lymphoma, comprising the steps of:
- (a) obtaining a blood sample from the patient;
- (b) isolating a predetermined population of cells from the blood sample, wherein the isolated cells are CD19+ and CD20+, using at least one of flow cytometry, fluorescence activated cell sorting, antibody-dependent depletion, antibody-dependent solid phase capture, panning, affinity column separation, and magnetic selection;
- (c) determining whether the isolated cells are ALDH;
- and
- (d) comparing the number of ALDH+ cells with the number of ALDH+ cells determined at an earlier time in the cancer treatment.
Dependencies are inferred from the claim text.