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US 8,741,640 B2Granted
Expanding hematopoietic stem cells
AssigneesHong Gao, Zhenglun Zhu
InventorsHong Gao, Zhenglun Zhu
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Abstract
A method of expanding hematopoietic stem cells. Also disclosed is a method of diagnosing primary or secondary bone marrow failure syndrome. The invention further includes a method of treating primary or secondary bone marrow failure syndrome.
Claims12
A method of expanding hematopoietic stem cells, the method comprising:
- isolating hematopoietic stem cells from a subject, and
- contacting the cells with an agent that inhibits expression or activity of a VentX polypeptide to increase expansion of the cells, wherein the agent is an iRNA agent having the sequence of SEQ ID NO:5, and
- incubating the cells thus contacted under conditions allowing expansion of the cells, whereby expansion of the cells is increased as compared to control cells.
A method of treating primary or secondary bone marrow failure syndrome, the method comprising:
- isolating hematopoietic stem cells from a subject having primary or secondary bone marrow failure syndrome,
- contacting the hematopoietic stem cells with an agent that inhibits expression or activity of a VentX polypeptide to increase expansion of the hematopoietic stem cells, wherein the agent is an siRNA agent having the sequence of SEQ ID NO:5,
- incubating the hematopoetic stem cells thus contacted under conditions allowing expansion of the hematopoetic stem cells, and
- administering to the subject an effective amount of the hematopoietic stem cells having undergone expansion.
1 more element
Dependencies are inferred from the claim text.