Microorganisms and processes for the production of isoprene
AssigneeGlycos Biotechnologies, Inc.
InventorsPaul Campbell, Sebastian Bredow, Huaijin Zhou, Stephanie Doneske, Daniel J. Monticello
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Abstract
The present invention provides a novel biosynthetic pathway for the production of isoprene from 3-methyl-2-buten-1-ol or 2-methyl-3-buten-2-ol. Further embodiments provide non-naturally occurring microorganism that have been modified to produce isoprene from 3-methyl-2-buten-1-ol or 2-methyl-3-buten-2-ol and methods of producing isoprene using said microorganism.
Claims20
A non-naturally occurring microbial organism comprising an isoprene biosynthetic pathway for the conversion of dimethylallyl diphosphate (DMAPP) to isoprene, wherein the isoprene biosynthetic pathway comprises the conversion of DMAPP to 2-methyl-3-buten-2-ol followed by the conversion of 2-methyl-3-buten-2-ol to isoprene and wherein the isoprene biosynthetic pathway comprises an exogenous nucleic acid encoding at least one 2-methyl-3-buten-2-ol synthase capable of converting DMAPP to 2-methyl-3-buten-2-ol and an exogenous nucleic acid encoding at least one 2-methyl-3-buten-2-ol dehydratase capable of converting 2-methyl-3-buten-2-ol to isoprene and wherein the isoprene biosynthetic pathway is expressed at a sufficient level to produce isoprene.
Dependencies are inferred from the claim text.