# US 8,739,736 B2 · Systems and methods for the portable electroanesthesia of fish

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- Number: US 8,739,736 B2
- Document ID: US8739736B2
- Status: Granted
- Kind: B2
- Application number: 12/582,579
- Filed: 2009-10-20
- Published: 2010-12-16
- Granted: 2014-06-03
- Earliest claimed: 2008-10-20
- Assignees: Smith-Root, Inc.
- Applicants: Farland Michael Holliman
- Inventors: Farland Michael Holliman
- Web page: https://patentgrep.com/patent/US8739736B2

## CPC

- A22B 3/06: Slaughtering or stunning by electric current (electric circuits therefor H05C)
- A22B: SLAUGHTERING

## Abstract

A portable fish anesthetizing system is also described having a tank cover, an electrode pair with the electrode pair configurable attached to the tank cover; a control system for creating a potential difference, the control system electrically connected to the electrode pair, so that when the tank cover is placed on a laboratory tank and the control system is activated, current flows from the control system through the electrodes.

## Claims

### Claim 1

A portable fish anesthetizing system comprising:
a tank cover,
an electrode pair, the electrode pair directly mechanically attached to the tank cover;
a control system for creating a potential difference, the control system electrically connected to the electrode pair;
a laboratory tank, the laboratory tank having a top rim, a bottom, and four upward walls connected to the bottom;
a camera removably positioned on at least one of the upward walls, the camera connected to the control system; so that when the camera is connected to the control system, a series of fish images are recorded in the tank, the control system then identifies a class of fish, and in response to the class of fish detected, thereby adjusts a current flow;
wherein activation of the current flow from the control system through the electrode pair creating a potential difference, occurs upon placement of the tank cover on the top rim of the laboratory tank, so that the fish experiences a physiological response; wherein the physiological response is at least one of electronarcosis, electrotaxis, and euthanasia.

### Claim 2 (depends on 1)

The portable fish anesthetizing system as in claim 1 wherein the laboratory tank has a volume that can hold from about 40 to about 60 quarts.

### Claim 3 (depends on 1)

The portable fish anesthetizing system as in claim 1 wherein the laboratory tank has dimensions of approximately 2 feet in width, 4 feet in length, and 2 feet in height.

### Claim 4 (depends on 1)

The portable fish anesthetizing system as in claim 1 wherein the potential difference between the electrode pair creates a field that induces electronarcosis in a laboratory fish.

### Claim 5 (depends on 1)

The portable fish anesthetizing system as in claim 1 wherein the potential difference between the electrode pair creates a potential field that induces electrotaxis in laboratory fish.

### Claim 6 (depends on 1)

The portable fish anesthetizing system as in claim 1 where the potential difference is a periodic waveform selected from a group consisting of:
(1) 100 Volt Pulse at 30 Hz with 25% duty cycle,
(2) 100 Volt Pulse at 60 Hz with 25% duty cycle,
(3) 100 Volt Pulse at 15 Hz with 25% duty cycle,
(4) 100 Volt Pulse at 95 Hz with 25% duty cycle,
(5) 100 Volt Pulse at 100 Hz with 25% duty cycle,
(6) burst of 3 100 Volt Pulses at 250 Hz with 62% duty cycle at 15 Hz,
(7) burst of 3 100 Volt Pulses at 500 Hz with 62% duty cycle at 30 Hz,
(8) burst of 3 50 Volt Pulses at 250 Hz with 62 duty at 15 Hz or 1 second followed by a burst of 3 100 volt pulses of 250 Hz with a 62% duty at 15 Hz for 2 seconds,
(9) burst of 3, 100 Volt Pulses at 250 Hz with 62% duty cycle at 15 Hz for 2 seconds followed by a burst of 3 50 Volt pulses of 250 Hz with 62% duty at 15 Hz for 1 second.

### Claim 7 (depends on 6)

The portable fish anesthetizing system as in claim 6 where the periodic waveform is a square wave.

### Claim 8 (depends on 6)

The portable fish anesthetizing system as in claim 6 wherein the periodic waveform has a positive phase and a negative phase, and wherein an amount of power generated in the positive phase and the negative phase are approximately equal.

### Claim 9 (depends on 6)

The portable fish anesthetizing system as in claim 6 where the potential difference ranges from 0 V to 200 V such that electronarcosis is induced in a laboratory fish.

### Claim 10 (depends on 9)

The portable fish anesthetizing system as in claim 9 where the laboratory fish is a zebrafish.

### Claim 11 (depends on 1)

The portable fish anesthetizing system as in claim 1 where the maximum potential difference ranges from 0 V to 200 V such that electrotaxis is induced in a laboratory fish.

### Claim 12 (depends on 11)

The portable fish anesthetizing system as in claim 11 where the laboratory fish is a zebrafish.
