Bacterially derived intact minicells that encompass plasmid free functional nucleic acid for in vivo delivery to mammalian cells
AssigneeEnGeneIC Molecular Delivery Pty Ltd
InventorsHimanshu Brahmbhatt, Jennifer MacDiarmid, Toby Hulf
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Abstract
Intact, bacterially-derived minicells can safely introduce therapeutically effective amounts of plasmid-free functional nucleic acid to target mammalian cells. To this end, functional nucleic acid can be packaged into intact minicells directly, without resort to expression constructs, the expression machinery of the host cell, harsh chemicals or electroporation.
Claims13
A method for formulating a minicell composition comprising (a) a plurality of intact, bacterially derived minicells, each minicell of said plurality encompassing plasmid-free regulatory RNA, and (b) a pharmaceutically acceptable carrier therefor, the method consisting essentially of co-incubating a plurality of intact, bacterially derived minicells with regulatory RNA in a saline buffer, wherein said regulatory RNA is selected from the group consisting of siRNA, miRNA and shRNA.
Dependencies are inferred from the claim text.