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US 8,735,134 B2Granted
Isoprene synthase variants with improved solubility for production of isoprene
AssigneesDanisco US Inc., The Goodyear Tire & Rubber Company
InventorsChristopher L. Rife, Derek H. Wells
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Abstract
The present invention provides methods and compositions of variant polypeptides having isoprene synthase activity with improved solubility. In particular, the present invention provides isoprene synthase variant for increased isoprene production in recombinant host cells.
Claims30
A recombinant host cell comprising a polynucleotide encoding an isolated polypeptide variant having isoprene synthase activity, wherein the polypeptide variant comprises a substitution at residue X288 corresponding to SEQ ID NO:
- 1, and wherein the polypeptide has increased protein solubility compared to a parent polypeptide which does not comprise the substitution at residue X288, wherein said polypeptide variant is 90% identical to isoprene synthase polypeptide of SEQ ID NO:
- 1.
Dependencies are inferred from the claim text.