Back to results
US 8,735,114 B2Granted
Fermentive production of four carbon alcohols
AssigneeButamax(Tm) Advanced Biofuels LLC
InventorsGail K Donaldson, Andrew C Eliot, Dennis Flint, Lori Ann Maggio-Hall, Vasantha Nagarajan
Life of this patent
Estimating the term…
Abstract
Methods for the fermentative production of four carbon alcohols is provided. Specifically, butanol, preferably isobutanol is produced by the fermentative growth of a recombinant bacterium expressing an isobutanol biosynthetic pathway.
Claims17
A method comprising:
- (a) providing a recombinant yeast host cell that expresses an engineered biosynthetic isobutanol pathway, wherein the engineered biosynthetic isobutanol pathway comprises an acetolactate synthase (ALS) enzyme, a ketol-acid reductoisomerase (KARI) enzyme, a dihydroxy-acid dehydratase (DHAD) enzyme, a branched chain keto acid decarboxylase enzyme, and an alcohol dehydrogenase (ADH) enzyme, each of which is encoded by a heterologous gene that lacks a mitochondrial targeting sequence, and wherein said yeast host cell is provided in a growth phase;
- (b) growing the yeast host cells in fermentation medium comprising suitable carbon substrates from renewable feedstocks, whereby isobutanol is bioproduced and wherein said cells grow during fermentation;
- (c) recovering the bioproduced isobutanol;
- and
- (d) blending the bioproduced isobutanol with a fossil fuel to make a fuel or fuel additive.
2 more elements
Dependencies are inferred from the claim text.