Lyase enzymes, nucleic acids encoding them and methods for making and using them

A method for deaminating a phenylalanine, tyrosine, or a histidine comprising the following steps:

• (a) providing a polypeptide having at least 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, 99%, or more, or 100% sequence identity to SEQ ID NO 32, or a fragment thereof, or a polypeptide encoded by a nucleic acid having at least 85%, 86%, 87%, 88%, 89%, 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, 99%, or more, or 100% sequence identity to SEQ ID NO 31;
• (b) providing a composition comprising a phenylalanine, tyrosine, or a histidine residue;
• and
• (c) contacting the polypeptide or the fragment thereof of step (a) with the composition of step (b) under conditions wherein the polypeptide or the fragment thereof deaminates the phenylalanine, tyrosine, or a histidine residue in the composition;
• wherein the polypeptide or the fragment thereof has lyase activity.