Recombinant microorganisms and uses thereof
OwnerAsis Datta, Sumit Ghosh +3inventors
InventorsAsis Datta, Sumit Ghosh, Swagata Ghosh, Hanumantha Rao Kongara, Mohan Kamthan
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Abstract
The present invention relates to recombinant strains of Vibrio spp, which are unable to utilize the amino sugar N-acetylglucosamine (GlcNAc) as a sole carbon source. This inability to utilize GlcNAc severely impairs the colonization property of the recombinants. The present invention also provides compositions comprising these recombinant strains for use in pharmaceuticals and in providing immunity.
Claims13
A recombinant strain of Vibrio cholerae incapable of utilizing N-acetylglucosamine (GlcNAc), wherein said recombinant strain comprises at least one mutation in at least one of the genes selected from the group consisting of N-acetylglucosamine-6-phosphate deacetylase (nagA1), N-acetylglucosamine-6-phosphate deacetylase II (nagA2), glucosamine 6-phosphate deaminase/isomerase (nagB), and GlcNAc specific transporter (nagE), wherein said recombinant strain shows impaired colonization in a host as compared to a wild type Vibrio cholerae.
Dependencies are inferred from the claim text.