Isolation of a virus related to canine parvovirus-2 from a raccoon

An immunogenic composition comprising a nucleic acid comprising the nucleotide sequence SEQ ID NO:

• 1, or a portion of SEQ ID NO:
• 1;
• wherein said portion of SEQ ID NO:
• 1 encodes an antigenic region of a VP-2 protein comprising both amino acid residue Thr at position 232 and amino acid residue Asp at position 300 of SEQ ID NO:
• 3, wherein said nucleic acid is present in a killed or attenuated parvovirus virion or low passage raccoon parvovirus (RPV).

A method of eliciting an immune response in an animal against parvovirus infection, comprising the step of administering to said animal an immunogenic composition comprising a nucleic acid comprising the nucleotide sequence SEQ ID NO:

• 1, or a portion of SEQ ID NO:
• 1, wherein said portion of SEQ ID NO:
• 1 encodes an antigenic region of a VP-2 protein comprising both amino acid residue Thr at position 232 and amino acid residue Asp at position 300 of SEQ ID NO:
• 3.

An expression vector comprising a nucleotide sequence encoding the amino acid sequence of SEQ ID NO:

• 3, or a portion of SEQ ID NO:
• 3;
• wherein said portion of SEQ ID NO:
• 3 is an antigenic region of a VP-2 protein comprising both amino acid residue Thr at position 232 and amino acid residue Asp at position 300 of SEQ ID NO:
• 3,
• wherein said expression vector is a recombinant expression vector or a killed or attenuated viral vector.