Sperm-specific cation channel, CATSPER2, and uses therefor
AssigneesChildren's Medical Center Corporation, Board of Regents, The University of Texas System
InventorsDejian Ren, David Clapham, David L. Garbers, Timothy A. Quill
Life of this patent
Estimating the term…
Abstract
Nucleic acid and protein sequences relating to a cation channel which is sperm-specific (CatSper2) are disclosed. The CatSper2 protein is shown to be specifically expressed in sperm. Nucleic acids, vectors, transformed cells, transgenic animals, polypeptides, and antibodies relating to the CatSper2 gene and protein are disclosed. Also provided are methods of in vitro fertilization and contraception, methods of identifying modulators of CatSper2 activity, methods of genotyping subjects with respect to CatSper2, methods of diagnosing and treating CatSper2-mediated disorders, including infertility, as well as methods of doing business related to CatSper2-mediated disorders.
Claims23
An isolated nucleic acid comprising:
- (a) a nucleotide sequence of SEQ ID NO:
- 1, or variants thereof that differ from SEQ ID NO:
- 1 by substitution of at least one synonymous codon, encoding the CatSper2 protein of SEQ ID NO:
- 2;
- or
- (b) a nucleotide sequence complementary to the nucleotide sequence of (a).
An isolated nucleic acid comprising:
- (a) a nucleotide sequence of SEQ ID NO:
- 5, or variants thereof that differ from SEQ ID NO:
- 5 by substitution of at least one synonymous codon, encoding the CatSper2 protein of SEQ ID NO:
- 6;
- or
- (b) a nucleotide sequence complementary to the nucleotide sequence of (a).
An isolated nucleic acid comprising:
- a nucleotide sequence encoding a polypeptide having at least 95% amino acid sequence identity with the CatSper2 protein of SEQ ID NO:
- 2 or 6,
- wherein the nucleotide sequence lacks introns and is derived from SEQ ID NO:
- 1 or 5, or variants thereof that differ from SEQ ID NO:
- 1 or 5 by substitution of at least one synonymous codon.
Dependencies are inferred from the claim text.