Methods for reducing the mitogenicity of lectin compositions
AssigneeSmartCells, Inc.
InventorsThomas M. Lancaster, Wenyi Cai, Ryan Faucette, Matthew Pecukonis, Todd C. Zion
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Abstract
Methods for reducing the T-cell mitogenicity of lectin compositions are provided. In one aspect this is achieved by chemically modifying mitogenic lectin compositions under optimized conditions. Additionally or alternatively, the reduction in T-cell mitogenicity is achieved by removing unmodified subunits chemically modified mixtures. Modified lectin compositions with reduced T-cell mitogenicity are also provided as are uses of the inventive compositions.
Claims24
A method of reducing the T-cell mitogenicity of a chemically modified lectin composition by affinity chromatography wherein the affinity chromatography is performed using beads in solution, wherein the method comprises:
- (a) providing a mitogenic lectin composition that includes a plurality of mitogenic lectins and reacting the mitogenic lectin composition with one or more chemical compounds to produce a chemically modified lectin composition;
- (b) contacting a chemically modified lectin composition with an immobilized ligand on the beads under conditions that allow at least a portion of the composition to bind the ligand;
- (c) exposing the bound portion of the chemically modified lectin composition to glucose at a first concentration;
- (d) exposing the remaining bound portion of the chemically modified lectin composition after step (c) to glucose at a second higher concentration;
- and
- (e) recovering the portion of the chemically modified lectin composition that is released at the second higher glucose concentration, wherein the T-cell mitogenicity of the recovered portion of the chemically modified lectin composition is less than the T-cell mitogenicity of the original chemically modified lectin composition.
Dependencies are inferred from the claim text.