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US 8,728,798 B2Granted
Biological methods for preparing adipic acid
AssigneeVerdezyne, Inc.
InventorsStephen Picataggio, Tom Beardslee
Life of this patent
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Abstract
The technology relates in part to biological methods for producing adipic acid and engineered microorganisms capable of such production.
Claims20
A method for producing adipic acid, comprising:
- (a) contacting a Candida yeast with a feedstock comprising a vegetable oil, wherein the Candida yeast comprises:
- (i) a disruption, deletion or knockout of (1) a polynucleotide that encodes a POX4 polypeptide or (2) a promoter operably linked to a polynucleotide that encodes a POX4 polypeptide, whereby POX4 activity is reduced or removed and POX5 activity is not reduced or removed,
- (ii) a disruption, deletion or knockout of (1) a polynucleotide that encodes a long chain acyl-CoA synthetase polypeptide or (2) a promoter operably linked to a polynucleotide that encodes a long chain acyl-CoA synthetase polypeptide, whereby a long chain acyl-CoA synthetase activity is reduced or removed, and
- (iii) a disruption, deletion or knockout of (1) a polynucleotide that encodes a long chain acyl-CoA synthetase polypeptide or (2) a promoter operably linked to a polynucleotide that encodes a long chain acyl-CoA synthetase polypeptide, whereby an acyl-CoA synthetase activity is reduced or removed;
- and
- (b) culturing the Candida yeast under conditions in which adipic acid is produced.
3 more elements
Dependencies are inferred from the claim text.