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US 8,712,699 B2Granted
Agglutination judgment method
AssigneeBeckman Coulter, Inc.
InventorTadashi Haga
Life of this patent
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Abstract
The present invention provides an agglutination judgment method includes a first step of collecting data of a transmitted light intensity from each of measuring points included in a predetermined region of the reaction pattern, a second step of calculating a parameter representing the dispersion of a transmitted light intensity in the region, and a third step of judging the agglutination or non-agglutination by comparing the parameter thus calculated with a predetermined reference value.
Claims5
An agglutination judgment method of judging a type of a pattern using an apparatus, comprising:
- obtaining an image of the pattern by a camera, the pattern being formed by particles in a liquid contained in a vessel, parts of the particles being gathered on a center region of a bottom of the vessel to form a first sub-pattern on the center region, remainders of the particles being distributed on a peripheral region of the bottom surrounding the center region to form second sub-patterns on the peripheral region, the peripheral region being divided from the center region by a boundary, each of the second sub-patterns having a smaller size than that of the first sub-pattern, and the apparatus comprising the camera, an image-processing circuit and a data-processing circuit;
- processing the image by the image-processing circuit to obtain a distribution of transmitted light intensity for only the peripheral region on which the second sub-patterns are formed;
- calculating a standard deviation for the distribution by the image-processing circuit;
- and
- comparing the standard deviation with a predetermined reference value by the data-processing circuit;
- wherein
- the first sub-pattern is formed with aggregate constituted by two or more of the particles and each of the second sub-patterns is constituted by two or more of the particles in the agglutination pattern;
- the first sub-pattern is formed with individual particles and each of the second sub-patterns is constituted by one of the particles in the non-agglutination pattern;
- and
- the first sub-pattern is formed with individual particles and each of the second sub-patterns is constituted by two or more of the particles in the partial agglutination pattern.
7 more elements
Dependencies are inferred from the claim text.