Automated measurement of concentration and/or amount in a biological sample

A computer program on a non-transitory computer readable medium comprising instructions to cause a computer to:

• measure a first color channel value in a plurality of pixels from a plurality of separate control samples, each separate control sample comprising a single color of interest;
• define a vector for each of the plurality of separate control samples, wherein each vector comprises an average of each first color channel value present in the plurality of separate control samples;
• define a matrix based upon control measurements comprising each of the averages for each of the first color channels;
• define a conversion matrix comprising the inverse of the matrix based upon the control measurements;
• measure second color channel values in an image of an experimental sample comprising a plurality of colors of interest, each pixel of the image of the experimental sample comprising a plurality of second color channels;
• calculate an amount of a color in the experimental sample by converting the second color channel values in the experimental sample using the conversion matrix;
• and
• outputting the amount of the color in the experimental sample.

A machine vision system for automated analysis of a biological sample on a slide comprising:

• a computer comprising:
• a system processor;
• a computer program on a nontransitory computer readable medium, the computer program comprising an image algorithm comprising instructions to cause the computer to:
• measure a first color channel value in a plurality of pixels from a plurality of separate control samples, each separate control sample comprising a single color of interest;
• define a vector for each of the plurality of separate control samples, wherein each vector comprises an average of each first color channel value present in the plurality of separate control samples;
• define a matrix comprising each of the averages for each of the color channels;
• define a conversion matrix comprising the inverse of the matrix based upon the control measurements;
• measure second color channel values in an image of an experimental sample comprising a plurality of colors of interest, each pixel of the image of the experimental sample comprising a plurality of second color channels;
• calculating an amount of a color in the experimental sample by converting the second color channel values in the experimental sample using the conversion matrix;
• and
• outputting the amount of a color in the experimental sample;
• a monitor in communication with the computer, and
• an input device in communication with the computer;
• an optical system in communication with the computer, comprising:
• a stage;
• an automated loading and unloading member for loading and unloading of a slide;
• an identification member;
• an optical sensing array in optical communication with the stage configured to acquire an image at a location on a slide and in electrical communication with the processor,
• a storage member for storing the location of a candidate object or area of interest;
• and
• a storage device for storing each image.