Back to results
US 8,710,429 B2Granted
Identification of biomarkers in biological samples and methods of using same
AssigneeThe Board of Regents of the University of OK
InventorsJay S. Hanas, James R. Hocker, Daniel J. Brackett, Russell G. Postier, Marvin D. Peyton, John Y. Cheung
Life of this patent
Estimating the term…
Abstract
The present invention is directed to methods of identifying biomarkers in liquid biological samples obtained from cancer patients or patients exhibiting a disease state. Such methods may include the use of electrospray ionization-time of flight mass spectrometry (ESI-TOF MS).
Claims29
A method of identifying biomarkers in liquid biological samples from cancer patients, comprising the steps of:
- (a) obtaining a plurality of first liquid biological samples, wherein the plurality of first liquid biological samples are obtained from patients having the same type of cancer;
- (b) obtaining a plurality of second liquid biological samples, wherein the plurality of second liquid biological samples are obtained from normal, healthy patients;
- (c) directly subjecting the plurality of first liquid biological samples and the plurality of second liquid biological samples to electrospray ionization mass spectrometry (ESI MS) to produce a mass spectrum profile for each liquid biological sample;
- (d) constructing a first database comprising peaks present in the mass spectrum profiles of the plurality of first liquid biological samples;
- (e) constructing a second database comprising peaks present in the mass spectrum profiles of the plurality of second liquid biological samples;
- (f) comparing the second database to the first database to identify at least one series of statistically significant peaks that distinguish the plurality of liquid biological samples obtained from cancer patients from the plurality of liquid biological samples obtained from healthy patients;
- and
- (g) determining that the at least one series of statistically significant peaks identified in (f) comprise at least one biomarker related to the type of cancer.
5 more elements
A method of identifying biomarkers in a liquid biological sample from a cancer patient, comprising the steps of:
- (a) obtaining a plurality of first liquid biological samples, wherein the plurality of first liquid biological samples are obtained from patients having the same type and subtype or stage of cancer;
- (b) obtaining a plurality of second liquid biological samples, wherein the plurality of second liquid biological samples are obtained from patients having the same type of cancer as the plurality of first liquid biological samples but a different subtype or stage of cancer;
- (c) obtaining a plurality of third liquid biological samples, wherein the plurality of third liquid biological samples are obtained from normal, healthy patients;
- (d) directly subjecting the plurality of first liquid biological samples, the plurality of second liquid biological samples and the plurality of third liquid biological samples to electrospray ionization mass spectrometry (ESI MS) to produce a mass spectrum profile for each liquid biological sample;
- (e) constructing a first database comprising peaks present in the mass spectrum profiles of the plurality of first liquid biological samples;
- (f) constructing a second database comprising peaks present in the mass spectrum profiles of the plurality of second liquid biological samples;
- (g) constructing a third database comprising peaks present in the mass spectrum profiles of the plurality of third liquid biological samples;
- (h) comparing the third database to the first and second databases to identify at least one series of statistically significant peaks that distinguish the plurality of liquid biological samples obtained from cancer patients from the plurality of liquid biological samples obtained from healthy patients;
- (i) determining that the at least one series of statistically significant peaks identified in (h) comprises at least one biomarker related to the type of cancer,
- (j) comparing the first database to the second database to identify at least one series of statistically significant peaks present in the plurality of liquid biological samples obtained from patients having one cancer subtype or stage that is not present in the plurality of liquid biological samples obtained from patients having another cancer subtype or stage;
- and
- (k) determining that the at least one series of statistically significant peaks identified in (j) comprises at least one biomarker related to a specific subtype or stage of cancer.
9 more elements
A method of identifying biomarkers in liquid biological samples from patients having a particular disease state, comprising the steps of:
- (a) obtaining a plurality of first liquid biological samples, wherein the plurality of first liquid biological samples are obtained from patients having a particular disease state;
- (b) obtaining a plurality of second liquid biological samples, wherein the plurality of second liquid biological samples are obtained from normal, healthy patients;
- (c) directly subjecting the plurality of first liquid biological samples and the plurality of second liquid biological samples to electrospray ionization mass spectrometry (ESI MS) to produce a mass spectrum profile for each liquid biological sample;
- (d) constructing a first database comprising peaks present in the mass spectrum profiles of the plurality of first liquid biological samples;
- (e) constructing a second database comprising peaks present in the mass spectrum profiles of the plurality of second liquid biological samples;
- (f) comparing the second database to the first database to identify at least one series of statistically significant peaks that distinguish the plurality of liquid biological samples obtained from patients having a particular disease state from the plurality of liquid biological samples obtained from healthy patients;
- and
- (g) determining that the at least one series of statistically significant peaks identified in (f) comprise at least one biomarker related to the type of particular disease state.
5 more elements
Dependencies are inferred from the claim text.