Method for producing male sterile plants using plant beclin 1/ATG6 expression
AssigneeCouncil of Scientific and Industrial Research
InventorsSamir Vishwanath Sawant, Rakesh Tuli, Sudhir Pratap Singh
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Abstract
The present invention relates to a method for producing male sterile plants. It involves selective killing of reproductive cells in plants by using an autophagy related gene plant BECLIN I/ATG6. An expression cassette comprising plant BECLIN I/ATG6, regulated by a tapetum specific promoter can induce killing of tapetum cells. A particular area of interest is transforming a plant with said genetic construct and expression of the plant BECLIN I/ATG6 gene in tapetum at early stage of anther development to cause early collapse of tapetum to produce nonviable pollen, thus imparting male sterility in plants.
Claims11
A method for producing a male sterile plant, comprising expressing a plant BECLIN 1/ATG6 gene in the tapetum layer of anthers during early stages of pollen development, thereby obtaining a male sterile plant.
A recombinant vector, comprising a chimeric gene fusion consisting of:
- i. an anther specific promoter, wherein the anther specific promoter is a tapetum specific promoter represented by SEQ ID NO:3;
- ii. a polynucleotide sequence that codes for a polypeptide having SEQ ID NO:2;
- and
- iii. a Nos terminator sequence.
A plant comprising a tapetum layer wherein the plant comprises a recombinant vector and wherein the recombinant vector comprises a chimeric gene fusion consisting of:
- i. an anther specific promoter, wherein the anther specific promoter is a tapetum specific promoter represented by SEQ ID NO:3;
- ii. a polynucleotide sequence that codes for a polypeptide having SEQ ID NO:2;
- and
- iii. a Nos terminator sequence.
A kit, comprising:
- a. a recombinant vector comprising a chimeric gene fusion consisting of:
- i. an anther specific promoter, wherein the anther specific promoter is a tapetum specific promoter represented by SEQ ID NO:3;
- ii. a polynucleotide sequence that codes for a polypeptide having SEQ ID NO:2;
- and
- iii. a Nos terminator sequence;
- b. suitable reagents;
- and
- c. instructions for a method for producing a male sterile plant, comprising expressing a plant BECLIN 1/ATG6 gene in the tapetum layer of anthers during early stages of pollen development, thereby obtaining a male sterile plant.
Dependencies are inferred from the claim text.