Processes for producing polyunsaturated fatty acids in transgenic organisms
AssigneeBASF Plant Science GmbH
InventorsJörg Bauer, Tom Wetjen
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Abstract
The present invention relates to polynucleotides from Ostreococcus lucimarinus which code for desaturases and elongases and which can be employed for the recombinant production of polyunsaturated fatty acids. The invention furthermore relates to vectors, host cells and transgenic nonhuman organisms which comprise the polynucleotides, and to the polypeptides encoded by the polynucleotides. Finally, the invention also relates to production processes for the polyunsaturated fatty acids and for oil, lipid and fatty acid compositions.
Claims22
An isolated polynucleotide comprising a nucleic acid sequence selected from the group consisting of:
- (a) the nucleic acid sequence of SEQ ID NO:
- 13;
- (b) a nucleic acid sequence which codes for a polypeptide comprising the amino acid sequence of SEQ ID NO:
- 14;
- (c) a nucleic acid sequence which codes for a polypeptide having at least 90% identity to a polypeptide which is encoded by the nucleic acid sequence of (a) or (b), where the polypeptide has Δ6-desaturase activity;
- and
- (d) a nucleic acid sequence comprising a fragment of the nucleic acid of (a), (b) or (c), where the fragment codes for a polypeptide with Δ6-desaturase activity,
- wherein the polynucleotide is comprised in a vector.
A transgenic host cell comprising an isolated polynucleotide comprising a nucleic acid sequence selected from the group consisting of:
- (a) the nucleic acid sequence of SEQ ID NO:
- 13;
- (b) a nucleic acid sequence which codes for a polypeptide comprising the amino acid sequence of SEQ ID NO:
- 14;
- (c) a nucleic acid sequence which codes for a polypeptide having at least 90% identity to a polypeptide which is encoded by the nucleic acid sequence of (a) or (b), where the polypeptide has Δ6-desaturase activity;
- and
- (d) a nucleic acid sequence comprising a fragment of the nucleic acid of (a), (b) or (c), where the fragment codes for a polypeptide with Δ6-desaturase activity.
A method of generating a polypeptide with Δ6-desaturase activity, comprising:
- (a) expressing in a host cell a nucleic acid sequence selected from the group consisting of:
- (i) the nucleic acid sequence of SEQ ID NO:
- 13;
- (ii) a nucleic acid sequence which codes for a polypeptide comprising the amino acid sequence of SEQ ID NO:
- 14;
- (iii) a nucleic acid sequence which codes for a polypeptide having at least 90% identity to a polypeptide which is encoded by the nucleic acid sequence of (a) or (b), where the polypeptide has Δ6-desaturase activity;
- and
- (iv) a nucleic acid sequence comprising a fragment of the nucleic acid of (a), (b) or (c), where the fragment codes for a polypeptide with Δ6-desaturase activity;
- and
- (b) obtaining, from the host cell, the polypeptide which is encoded by the nucleic acid sequence.
A polypeptide which is encoded by a nucleic acid sequence selected from the group consisting of:
- (i) the nucleic acid sequence of SEQ ID NO:
- 13;
- (ii) a nucleic acid sequence which codes for a polypeptide comprising the amino acid sequence of SEQ ID NO:
- 14;
- (iii) a nucleic acid sequence which codes for a polypeptide having at least 90% identity to a polypeptide which is encoded by the nucleic acid sequence of (a) or (b), where the polypeptide has Δ6-desaturase activity;
- and
- (iv) a nucleic acid sequence comprising a fragment of the nucleic acid of (a), (b) or (c), where the fragment codes for a polypeptide with Δ6-desaturase activity.
A transgenic microorganism comprising an isolated polynucleotide comprising a nucleic acid sequence selected from the group consisting of:
- (a) the nucleic acid sequence of SEQ ID NO:
- 13;
- (b) a nucleic acid sequence which codes for a polypeptide comprising the amino acid sequence of SEQ ID NO:
- 14;
- (c) a nucleic acid sequence which codes for a polypeptide having at least 90% identity to a polypeptide which is encoded by the nucleic acid sequence of (a) or (b), where the polypeptide has Δ6-desaturase activity;
- and
- (d) a nucleic acid sequence comprising a fragment of the nucleic acid of (a), (b) or (c), where the fragment codes for a polypeptide with Δ6-desaturase activity.
An isolated polynucleotide comprising a nucleic acid sequence selected from the group consisting of:
- (a) the nucleic acid sequence of SEQ ID NO:
- 13;
- (b) a nucleic acid sequence which codes for a polypeptide comprising the amino acid sequence of SEQ ID NO:
- 14;
- and
- (c) a nucleic acid sequence which codes for a polypeptide comprising an amino acid sequence having at least 90% sequence identity with the sequence of SEQ ID NO:
- 14, where the polypeptide has Δ6-desaturase activity,
- wherein the polynucleotide is comprised in a vector.
Dependencies are inferred from the claim text.