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AssigneeUniversity of Florida Research Foundation, Inc.
InventorsL. Curtis Hannah, Carla R. Lyerly Linebarger
Estimating the term…
The subject invention concerns polynucleotides encoding a small subunit of plant AGP having one or more mutations in the amino acid sequence wherein the mutation confers increased heat stability to the expressed AGP enzyme. Mutations in the N-terminus of the small subunit of heat labile plant AGP results in AGP enzymes that are significantly more heat stable compared to wild type AGP in that the mutant AGP retains significant levels of enzymatic activity following exposure to heat treatment. In one embodiment, the polynucleotide encodes a mutant small subunit of maize AGP. The subject invention also concerns methods for providing a plant with increased resistance to heat conditions. Plants with heat labile AGP can be transformed with a polynucleotide of the present invention. The subject invention also concerns these transformed plants and transgenic progeny thereof. The subject invention also concerns mutant polypeptides encoded by polynucleotides of the present invention.
A polynucleotide comprising a polynucleotide sequence encoding a mutant small subunit of a heat labile plant ADP glucose pyrophosphorylase (AGP) enzyme, or a fragment thereof, wherein said small subunit, or said fragment thereof, comprises a mutation in the N-terminal portion thereof wherein the amino acid corresponding to tyrosine at position 36 of wild type maize endosperm small subunit sequence is replaced by a cysteine, and wherein when said mutant small subunit, or said fragment thereof, is expressed with a large subunit of a plant AGP enzyme to form a mutant enzyme, said mutant enzyme exhibits increased heat stability when compared to a wild type form of maize endosperm AGP enzyme.
A plant, plant tissue or cell comprising a polynucleotide comprising a polynucleotide sequence encoding a mutant small subunit of a heat labile plant ADP glucose pyrophosphorylase (AGP) enzyme, or a fragment thereof, wherein said small subunit, or said fragment thereof, comprises a mutation in the N-terminal portion thereof wherein the amino acid corresponding to tyrosine at position 36 of wild type maize endosperm small subunit sequence is replaced by a cysteine, and wherein when said mutant small subunit, or said fragment thereof, is expressed with a large subunit of a plant AGP enzyme to form a mutant enzyme, said mutant enzyme exhibits increased heat stability when compared to a wild type form of maize endosperm AGP enzyme.
A composition comprising:
An expression construct comprising a polynucleotide comprising a polynucleotide sequence encoding a mutant small subunit of a heat labile plant ADP glucose pyrophosphorylase (AGP) enzyme, or a fragment thereof, wherein said small subunit, or said fragment thereof, comprises a mutation in the N-terminal portion thereof wherein the amino acid corresponding to tyrosine at position 36 of wild type maize endosperm small subunit sequence is replaced by a cysteine, and wherein when said mutant small subunit, or said fragment thereof, is expressed with a large subunit of a plant AGP enzyme to form a mutant enzyme, said mutant enzyme exhibits increased heat stability when compared to a wild type form of maize endosperm AGP enzyme.
Dependencies are inferred from the claim text.