Compositions, organisms, systems, and methods for expressing a gene product in plants using SCBV expression control sequences operable in monocots and dicots

An expression vector comprising, in a 5′ to 3′ direction:

• a sugarcane bacilliform virus (SCBV) promoter having the sequence of SEQ ID NO:
• 1;
• an expressible nucleic acid;
• and a 3′ termination sequence, wherein the SCBV promoter has promoter activity sufficient to express the expressible nucleic acid in at least one monocot and at least one dicot, and wherein the expressible nucleic acid is heterologous to the sequence of SEQ ID NO:
• 1.

A bacterial cell comprising an expression vector having:

• a SCBV promoter having a sequence selected from the group consisting of the sequence of nucleotides 1-1786 of SEQ ID NO:
• 1, the sequence of SEQ ID NO:
• 1, the sequence of SEQ ID NO:
• 17, the sequence of SEQ ID NO:
• 26, and the sequence of SEQ ID NO:
• 27;
• an expressible nucleic acid;
• and
• a 3′ termination sequence, wherein the SCBV promoter has promoter activity sufficient to express the expressible exogenous nucleic acid in at least one monocot and at least one dicot.

A plant cell comprising an expression vector having:

• a promoter having a SCBV sequence selected from the group consisting of the sequence of nucleotides 1-1786 of SEQ ID NO:
• 1, the sequence of SEQ ID NO:
• 1, the sequence of SEQ ID NO:
• 17, the sequence of SEQ ID NO:
• 26, and the sequence of SEQ ID NO:
• 27;
• an expressible nucleic acid operably linked to the promoter;
• and a 3′ termination sequence,
• wherein the promoter has promoter activity sufficient to express the expressible nucleic acid in at least one monocot and at least one dicot, and wherein the expressible nucleic acid is heterologous to the sequence of the promoter.

A plant comprising an expression vector having:

• a promoter having a SCBV sequence-selected from the group consisting of the sequence of nucleotides 1-1786 of SEQ ID NO:
• 1, the sequence of SEQ ID NO:
• 1, the sequence of SEQ ID NO:
• 17, the sequence of SEQ ID NO:
• 26, and the sequence of SEQ ID NO:
• 27;
• an expressible nucleic acid operably linked to the promoter;
• and
• a 3′ termination sequence,
• wherein the promoter has promoter activity sufficient to express the expressible nucleic acid in at least one monocot and at least one dicot, and wherein the expressible nucleic acid is heterologous to the sequence of the promoter.

A method for constitutively expressing an expressible nucleic acid in a plant, the method comprising:

• contacting an expression cassette or expression vector with the cytosol of a cell of the plant,
• wherein the expression cassette or expression vector comprises (i) the expressible nucleic acid, (ii) a SCBV promoter comprising a SCBV sequence-selected from the group consisting of the sequence of nucleotides 1-1786 of SEQ ID NO:
• 1, the sequence of SEQ ID NO:
• 1, the sequence of SEQ ID NO:
• 17, the sequence of SEQ ID NO:
• 26, and the sequence of SEQ ID NO:
• 27 and operable to drive expression of the expressible nucleic acid, and (iii) a 3′ termination sequence operably linked to the expressible nucleic acid, and
• wherein the plant is selected from the group consisting of a monocot and a dicot.

A method of directing constitutive expression of a nucleic acid in a plant comprising:

• transforming the plant with an expression nucleic acid, the expression nucleic acid comprising a promoter having its sequence selected from the group consisting of the sequence of nucleotides 1-1786 of SEQ ID NO:
• 1, the sequence of SEQ ID NO:
• 1, the sequence of SEQ ID NO:
• 17, the sequence of SEQ ID NO:
• 26, and the sequence of SEQ ID NO:
• 27, an expressible nucleic acid, and a 3′ termination sequence,
• wherein the plant is selected from the group consisting of a monocot and a dicot.

An isolated nucleic acid comprising in a 5′ to 3′ direction:

• (a) an expression control sequence, having a 3′ end and having promoter activity in at least one monocot and at least one dicot, and
• (b) an expressible nucleic acid having a 5′ end,
• wherein
• the expression control sequence comprises the sequence of nucleotides 1-1786 of SEQ ID NO:
• 18,
• the 3′ end of the expression control sequence and the 5′ end of the expressible nucleic acid together comprise the nucleotide sequence AAAATGG, and
• the expressible nucleic acid is heterologous to the expression control sequence.

An isolated nucleic acid comprising:

• (a) an expression control sequence having promoter activity in at least one monocot and at least one dicot, wherein the expression control sequence is selected from the group consisting of the sequence of SEQ ID NO:
• 18, the sequence of SEQ ID NO:
• 26, the sequence of SEQ ID NO:
• 27, the sequence of SEQ ID NO:
• 32, and the sequence of SEQ ID NO:
• 33, and
• (b) an expressible exogenous nucleic acid,
• wherein the expression control sequence has promoter activity in at least one monocot and at least one dicot and the expressible nucleic acid is heterologous to the expression control sequence.