Soybean EF1A promoter and its use in constitutive expression of transgenic genes in plants
AssigneeE. I. Du Pont de Nemours and Company
InventorZhongsen Li
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Abstract
The promoter of a soybean translation elongation factor EF1 alpha, a polypeptide that promotes the GTP-dependent binding of aminoacyl-tRNA to the A-site of ribosomes during protein biosynthesis, and fragments thereof and their use in promoting the expression of one or more heterologous nucleic acid fragments in a tissue-independent or constitutive manner in plants are described.
Claims18
A recombinant DNA construct comprising
- a nucleotide sequence comprising any one of the sequences set forth in SEQ ID NO:1, SEQ ID NO:2, SEQ ID NO:3, SEQ ID NO:4, SEQ ID NO:5, SEQ ID NO:6 or SEQ ID NO:7,
- operably linked to at least one heterologous sequence,
- wherein said nucleotide sequence has constitutive promoter activity.
A method for expressing a yellow fluorescent protein ZS-YELLOW1 N1 in a host cell comprising:
- (a) transforming a host cell with a recombinant expression construct comprising at least one ZS-YELLOW1 N1 (YFP) nucleic acid fragment operably linked to a promoter wherein said promoter consists of the nucleotide sequence set forth in SEQ ID NOs:
- 1 or 3;
- and
- (b) growing the transformed host cell under conditions that are suitable for expression of the recombinant DNA construct, wherein expression of the recombinant DNA construct results in production of increased levels of ZS-YELLOW1 N1 protein in the transformed host cell when compared to a corresponding nontransformed host cell.
A plant stably transformed with a recombinant expression construct comprising a soybean promoter and a heterologous nucleic acid fragment operably linked to said promoter, wherein said promoter is a capable of controlling expression of said heterologous nucleic acid fragment in a plant cell, and further wherein said promoter comprises any one of the sequences set forth in SEQ ID NO:1, SEQ ID NO:2, SEQ ID NO:3, SEQ ID NO:4, SEQ ID NO:5, SEQ ID NO:6 or SEQ ID NO:7.
Dependencies are inferred from the claim text.