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US 8,710,203 B2Granted
Therapeutic human anti-IL-1R1 monoclonal antibody
AssigneesAmgen Inc., Medarex, Inc.
InventorsBrian Varnum, Chris Vezina, Alison Witte, Xueming Qian, Francis Hall Martin, Haichun Huang, Gary Elliot
Life of this patent
Estimating the term…
Abstract
Antibodies that interact with interleukin-1 receptor type 1 (IL-1R1) are described. Methods of treating IL-1 mediated diseases by administering a pharmaceutically effective amount of antibodies to IL-1R1 are described. Methods of detecting the amount of IL-1R1 in a sample using antibodies to IL-1R1 are described.
Claims24
An isolated nucleic acid molecule comprising:
- (a) nucleotide sequences encoding human heavy chain framework regions, a nucleotide sequence encoding a human heavy chain CDR1 region comprising SEQ ID NO:
- 61, a nucleotide sequence encoding a human heavy chain CDR2 region comprising SEQ ID NO:
- 64, and a nucleotide sequence encoding a human heavy chain CDR3 region comprising SEQ ID NO:
- 67;
- and nucleotide sequences encoding human light chain framework regions, a nucleotide sequence encoding a human light chain CDR1 region comprising SEQ ID NO:
- 70, a nucleotide sequence encoding a human light chain CDR2 region comprising SEQ ID NO:
- 72, and a nucleotide sequence encoding a human light chain CDR3 region comprising SEQ ID NO:
- 74;
- or
- (b) nucleotide sequences encoding human heavy chain framework regions, a nucleotide sequence encoding a human heavy chain CDR1 region comprising SEQ ID NO:
- 62, a nucleotide sequence encoding a human heavy chain CDR2 region comprising SEQ ID NO:
- 65, and a nucleotide sequence encoding a human heavy chain CDR3 region comprising SEQ ID NO:
- 68;
- and nucleotide sequences encoding human light chain framework regions, a nucleotide sequence encoding a human light chain CDR1 region comprising SEQ ID NO:
- 70, a nucleotide sequence encoding a human light chain CDR2 region comprising SEQ ID NO:
- 72, and a nucleotide sequence encoding a human light chain CDR3 region comprising SEQ ID NO:
- 74.
14 more elements
An isolated nucleic acid molecule comprising a nucleotide sequence that encodes a human antibody that specifically binds IL-1R1, wherein the antibody comprises:
- (a) a heavy chain having a heavy chain variable region, or an antigen-binding fragment thereof, wherein the heavy chain variable region comprises the amino acid sequence encoded by SEQ ID NO:
- 89, and wherein the antigen-binding fragment comprises an N-terminal or C-terminal deletion of the amino acid sequence encoded by SEQ ID NO:
- 89, wherein the N-terminal or C-terminal deletion comprises SEQ ID NO:
- 61, SEQ ID NO:
- 64, and SEQ ID NO:
- 67;
- or
- (b) a heavy chain having a heavy chain variable region, or an antigen-binding fragment thereof, wherein the heavy chain variable region comprises the amino acid sequence encoded by SEQ ID NO:
- 87, and wherein the antigen-binding fragment comprises an N-terminal or C-terminal deletion of the amino acid sequence encoded by SEQ ID NO:
- 87, wherein the N-terminal or C-terminal deletion comprises SEQ ID NO:
- 62, SEQ ID NO:
- 65, and SEQ ID NO:
- 68.
10 more elements
An isolated nucleic acid molecule comprising a nucleotide sequence that encodes a human antibody that specifically binds IL-1R1, wherein the antibody comprises:
- (a) a heavy chain having a heavy chain variable region, or an antigen-binding fragment thereof, wherein the heavy chain variable region comprises the amino acid sequence encoded by SEQ ID NO:
- 89, and wherein the antigen-binding fragment comprises SEQ ID NO:
- 61, SEQ ID NO:
- 64, and SEQ ID NO:
- 67, and wherein the antibody comprises a light chain having a light chain variable region, or an antigen-binding fragment thereof, wherein the light chain variable region comprises the amino acid sequence encoded by SEQ ID NO:
- 88, and wherein the antigen-binding fragment comprises SEQ ID NO:
- 70, SEQ ID NO:
- 72, and SEQ ID NO:
- 74;
- or
- (b) a heavy chain having a heavy chain variable region, or an antigen-binding fragment thereof, wherein the heavy chain variable region comprises the amino acid sequence encoded by SEQ ID NO:
- 87, and wherein the antigen-binding fragment comprises SEQ ID NO:
- 62, SEQ ID NO:
- 65, and SEQ ID NO:
- 68, and wherein the antibody comprises a light chain having a light chain variable region, or an antigen-binding fragment thereof, wherein the light chain variable region comprises the amino acid sequence encoded by SEQ ID NO:
- 88, and wherein the antigen-binding fragment comprises SEQ ID NO:
- 70, SEQ ID NO:
- 72, and SEQ ID NO:
- 74.
16 more elements
A set of nucleic acid molecules comprising:
- (a) a first nucleic acid molecule encoding human heavy chain framework regions, a nucleotide sequence encoding a human heavy chain CDR1 region comprising SEQ ID NO:
- 61, a nucleotide sequence encoding a human heavy chain CDR2 region comprising SEQ ID NO:
- 64, and a nucleotide sequence encoding a human heavy chain CDR3 region comprising SEQ ID NO:
- 67;
- and a second nucleic acid molecule encoding human light chain framework regions, a nucleotide sequence encoding a human light chain CDR1 region comprising SEQ ID NO:
- 70, a nucleotide sequence encoding a human light chain CDR2 region comprising SEQ ID NO:
- 72, and a nucleotide sequence encoding a human light chain CDR3 region comprising SEQ ID NO:
- 74;
- or
- (b) a first nucleic acid molecule encoding human heavy chain framework regions, a nucleotide sequence encoding a human heavy chain CDR1 region comprising SEQ ID NO:
- 62, a nucleotide sequence encoding a human heavy chain CDR2 region comprising SEQ ID NO:
- 65, and a nucleotide sequence encoding a human heavy chain CDR3 region comprising SEQ ID NO:
- 68;
- and a second nucleic acid molecule encoding human light chain framework regions, a nucleotide sequence encoding a human light chain CDR1 region comprising SEQ ID NO:
- 70, a nucleotide sequence encoding a human light chain CDR2 region comprising SEQ ID NO:
- 72, and a nucleotide sequence encoding a human light chain CDR3 region comprising SEQ ID NO:
- 74.
14 more elements
A set of nucleic acid molecules comprising nucleotide sequences that encode a human antibody that specifically binds IL-1R1, comprising:
- (a) a first nucleic acid molecule encoding a human antibody heavy chain having a heavy chain variable region, or an antigen-binding fragment thereof, wherein the heavy chain variable region comprises the amino acid sequence encoded by SEQ ID NO:
- 9, and wherein the antigen-binding fragment comprises an N-terminal or C-terminal deletion of the amino acid sequence encoded by SEQ ID NO:
- 9, wherein the N-terminal or C-terminal deletion comprises SEQ ID NO:
- 61, SEQ ID NO:
- 64, and SEQ ID NO:
- 67;
- and a second nucleic acid molecule encoding a human antibody light chain;
- or
- (b) a first nucleic acid molecule encoding a human antibody heavy chain having a heavy chain variable region, or an antigen-binding fragment thereof, wherein the heavy chain variable region comprises the amino acid sequence encoded by SEQ ID NO:
- 13, and wherein the antigen-binding fragment comprises an N-terminal or C-terminal deletion of the amino acid sequence encoded by SEQ ID NO:
- 13, wherein the N-terminal or C-terminal deletion comprises SEQ ID NO:
- 62, SEQ ID NO:
- 65, and SEQ ID NO:
- 68;
- and a second nucleic acid molecule encoding a human antibody light chain.
12 more elements
A set of nucleic acid molecules comprising:
- (a) a first nucleic acid molecule encoding a human antibody heavy chain having a heavy chain variable region, or an antigen-binding fragment thereof, wherein the heavy chain variable region comprises the amino acid sequence encoded by SEQ ID NO:
- 89, and wherein the antigen-binding fragment comprises SEQ ID NO:
- 61, SEQ ID NO:
- 64, and SEQ ID NO:
- 67;
- and a second nucleic acid molecule encoding a human antibody light chain having a light chain variable region, or an antigen-binding fragment thereof, wherein the light chain variable region comprises the amino acid sequence encoded by SEQ ID NO:
- 88, and wherein the antigen-binding fragment comprises SEQ ID NO:
- 70, SEQ ID NO:
- 72, and SEQ ID NO:
- 74;
- or
- (b) a first nucleic acid molecule encoding a human antibody heavy chain having a heavy chain variable region, or an antigen-binding fragment thereof, wherein the heavy chain variable region comprises the amino acid sequence encoded by SEQ ID NO:
- 87, and wherein the antigen-binding fragment comprises SEQ ID NO:
- 62, SEQ ID NO:
- 65, and SEQ ID NO:
- 68;
- and a second nucleic acid molecule encoding a human antibody light chain having a light chain variable region, or an antigen-binding fragment thereof, wherein the light chain variable region comprises the amino acid sequence encoded by SEQ ID NO:
- 88, and wherein the antigen-binding fragment comprises SEQ ID NO:
- 70, SEQ ID NO:
- 72, and SEQ ID NO:
- 74.
18 more elements
A set of nucleic acid molecules comprising:
- (a) a first nucleic acid molecule comprising SEQ ID NO:
- 89;
- and a second nucleic acid molecule comprising SEQ ID NO:
- 88;
- or
- (b) a first nucleic acid molecule comprising SEQ ID NO:
- 87;
- and a second nucleic acid molecule comprising SEQ ID NO:
- 88.
6 more elements
Dependencies are inferred from the claim text.