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US 8,710,196 B2Granted
Protein purification
AssigneeGenentech, Inc.
InventorsJefferson C. Emery, Paul J. McDonald, Rhona O'Leary
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Abstract
A method for purifying a polypeptide by ion exchange chromatography is described in which a gradient wash is used to resolve a polypeptide of interest from one or more contaminants.
Claims14
A method for purifying an antibody from a composition comprising the antibody and a contaminant, which method comprises the following steps performed sequentially:
- (a) binding the antibody to a cation exchange material with an equilibration buffer at a first conductivity;
- (b) washing the cation exchange material with a wash buffer, wherein the conductivity of the wash buffer increases from a second conductivity that is higher than the first conductivity to a third conductivity during the washing;
- (c) passing a fixed volume of wash buffer at the third conductivity over the cation exchange material;
- and
- (d) eluting the antibody from the cation exchange material with an elution buffer at a fourth conductivity that is higher than the third conductivity.
2 more elements
Dependencies are inferred from the claim text.