Single-chain variable fragment (scFv) able to recognize and bind CD99 human protein
AssigneeIstituto Ortopedico Rizzoli
InventorsPiero Picci, Katia Scotlandi, Alessandro Ascione, Maurizio Cianfriglia, Maria Luisa Dupuis, Michela Flego, Mara Gellini, Alessandra Mallano
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Abstract
The present invention relates to a single-chain variable fragment (scFv) able to recognize an epitope of the extracellular domain of CD99 human protein. The single-chain variable fragment is able to recognize and specifically and selectively bind the epitope of the extracellular domain of CD99 human protein expressed on Ewing sarcoma cells. The fragment can thus be used for the diagnosis and 10 treatment of Ewing sarcoma.
Claims26
A single-chain variable fragment (scFv), comprising:
- a heavy variable chain (VH);
- a light variable chain (VL);
- and
- a peptide linker joining said heavy variable chain to said light variable chain, wherein said fragment is directed against an epitope of the extracellular domain of the CD99 human protein consisting essentially of the amino acid sequence SEQ ID NO:
- 25 and wherein the VH chain comprises CDR1, CDR2 and CDR3 regions consisting essentially of amino acid sequences SEQ ID NO:
- 1, SEQ ID NO:
- 3 and SEQ ID NO:
- 4, respectively and the VL chain comprises CDR1, CDR2 and CDR3 regions consisting essentially of amino acid sequences SEQ ID NO:
- 2, SEQ ID NO:
- 5 and SEQ ID NO:
- 6, respectively.
A human, humanized or chimeric antibody directed against an epitope of the extracellular domain of the CD99 human protein consisting essentially of the amino acid sequence SEQ ID NO:
- 25 wherein said antibody has the VH chain comprising CDR1, CDR2 and CDR3 regions consisting essentially of amino acid sequences SEQ ID NO:
- 1, SEQ ID NO:
- 3 and SEQ ID NO:
- 4, respectively and the VL chain comprising CDR1, CDR2 and CDR3 regions consisting essentially of amino acid sequences SEQ ID NO:
- 2, SEQ ID NO:
- 5 and SEQ ID NO:
- 6, respectively.
An antibody fragment directed against an epitope of the extracellular domain of the CD99 human protein consisting essentially of the amino acid sequence SEQ ID NO:
- 25 and wherein said fragment has the VH chain comprising CDR1, CDR2 and CDR3 regions consisting essentially of amino acid sequences SEQ ID NO:
- 1, SEQ ID NO:
- 3 and SEQ ID NO:
- 4, respectively and the VL chain comprising CDR1, CDR2 and CDR3 regions consisting essentially of amino acid sequences SEQ ID NO:
- 2, SEQ ID NO:
- 5 and SEQ ID NO:
- 6, respectively.
A diabody directed against an epitope of the extracellular domain of the CD99 human protein consisting essentially of the amino acid sequence SEQ ID NO:
- 25 and having a VH chain with CDR1, CDR2 and CDR3 regions consisting essentially of amino acid sequences SEQ ID NO:
- 1, SEQ ID NO:
- 3 and SEQ ID NO:
- 4, respectively and a VL chain with CDR1, CDR2 and CDR3 regions consisting essentially of amino acid sequences SEQ ID NO:
- 2, SEQ ID NO:
- 5 and SEQ ID NO:
- 6, respectively.
Dependencies are inferred from the claim text.