VEGF-specific capture agents, compositions, and methods of using and making
AssigneeIndi Molecular, Inc.
InventorsSuresh Mark Pitram, Heather Dawn Agnew, Tsun Yin Lai, Rosemary Dyane Rohde, Paul Edward Kearney
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Abstract
The present application provides biligand and triligand protein-catalyzed capture (PCC) agents that specifically bind VEGF, as well as the use of these capture agents as detection, diagnosis, and treatment agents.
Claims35
A stable, synthetic capture agent that specifically binds VEGF, wherein the capture agent comprises a designed anchor ligand, a designed secondary ligand, and, optionally, a designed tertiary ligand, and wherein the anchor ligand and secondary ligand selectively bind VEGF, wherein the anchor ligand comprises an amino acid sequence 95% identical to the amino acid sequence of SEQ ID NO:1 and wherein the secondary ligand comprises a formula of X2-X3-X4-X5-X6, wherein X2 is selected from the group consisting of D-arginine, D-tryptophan, D-leucine, D-valine, glycine, D-proline, D-isoleucine and D-alanine;
- wherein X3 is selected from the group consisting of D-proline, D-phenylalanine, glycine, D-arginine, D-lysine, D-histidine, D-tryptophan and D-tyrosine;
- wherein X4 is selected from the group consisting of D-isoleucine, D-leucine, D-proline, D-alanine, D-arginine, D-phenylalanine, D-threonine and D-histidine;
- wherein X5 is selected from the group consisting of D-isoleucine, glycine, D-valine, D-leucine, D-alanine, D-proline, D-lysine, D-glutamate, D-histidine and D-arginine;
- and wherein X6 is selected from the group consisting of D-arginine, D-lysine, D-tryptophan, D-tyrosine, D-proline, D-valine, glycine, D-isoleucine, D-alanine and D-glutamine.
Dependencies are inferred from the claim text.