Compositions and methods for concentrating and depleting microorganisms
AssigneeTechnion Research & Development Foundation Limited
InventorsAmram Mor, Shahar Rotem, Yechezkel Kashi, Nili Raz
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Abstract
Methods for concentrating microorganisms in a liquid sample or depleting microorganisms therefrom, utilizing polymeric compounds having affinity to microbial cells that are composed of a plurality of positively charged amino acid residues and two or more hydrophobic moieties are disclosed. Also disclosed are devices for concentrating and methods for detection and identification microorganisms in a liquid sample.
Claims39
A method of concentrating viable microorganisms in an aqueous solution, comprising contacting the solution with a water-insoluble matrix having a polymer capable of binding the microorganism covalently attached thereto via a C-terminus thereof, said polymer comprises a plurality of residues, wherein said plurality of residues comprises a plurality of amino acid residues and 3, 4, 5, 6, 7 or 8 ω-amino-fatty acid residues each being independently selected from the 10-amino-capric acid, 12-amino-lauric acid, 14-amino-myristic acid, 16-amino-palmitic acid, 18-amino-stearic acid, 18-amino-oleic acid, 16-amino-palmitoleic acid, 18-amino-linoleic acid, 18-amino-linolenic acid and 20-amino-arachidonic acid, whereas each of said ω-amino-fatty acid residues is being covalently linked to at least two amino acid residues in said plurality of amino acid residues via an amine group of one amino acid residue and via a carboxyl of the other amino acid residue in said at least two amino acid residues, said polymer being selected from the group consisting of a linear polymer and a cyclic polymer, thereby binding the microorganisms to said polymer on said matrix and concentrating the microorganisms in the aqueous solution, wherein said binding is effected while maintaining viability of the microorganisms.
A method of depleting microorganisms from an aqueous solution, the method comprising:
- (a) contacting the solution with a water-insoluble matrix having a polymer capable of binding the microorganism covalently attached thereto via a C-terminus thereof, wherein said polymer comprises a plurality of residues, and wherein said plurality of residues comprises a plurality of amino acid residues and 3, 4, 5, 6, 7 or 8 ω-amino-fatty acid residues each being independently selected from the group consisting of 10-amino-capric acid, 12-amino-lauric acid, 14-amino-myristic acid, 16-amino-palmitic acid, 18-amino-stearic acid, 18-amino-oleic acid, 16-amino-palmitoleic acid, 18-amino-linoleic acid, 18-amino-linolenic acid and 20-amino-arachidonic acid, whereas each of said ω-amino-fatty acid residues is being covalently linked to at least two amino acid residues in said plurality of amino acid residues via an amine group of one amino acid residue and via a carboxyl of the other amino acid residue in said at least two amino acid residues, said polymer being selected from the group consisting of a linear polymer and a cyclic polymer, thereby binding the microorganisms to said polymer on said matrix, wherein said binding is effected while maintaining viability of the microorganisms;
- and subsequently
- (b) collecting the solution depleted from the microorganisms.
A device for concentrating microorganisms while maintaining viability of the microorganisms, the device comprising at least one casing and a water-insoluble matrix embedded therein, said water insoluble matrix having a polymer capable of binding the microorganism covalently attached thereto via a C-terminus thereof, said polymer comprises a plurality of residues, wherein said plurality of residues comprises a plurality of amino acid residues and 3, 4, 5, 6, 7 or 8 ω-amino-fatty acid residues each being independently selected from the group consisting of 10-amino-capric acid, 12-amino-lauric acid, 14-amino-myristic acid, 16-amino-palmitic acid, 18-amino-stearic acid, 18-amino-oleic acid, 16-amino-palmitoleic acid, 18-amino-linoleic acid, 18-amino-linolenic acid and 20-amino-arachidonic acid, whereas each of said ω-amino-fatty acid residues is covalently linked to at least two amino acid residues in said plurality of amino acid residues via an amine group of one amino acid residue and via a carboxyl of the other amino acid residue in said at least two amino acid residues, said polymer being selected from the group consisting of a linear polymer and a cyclic polymer, wherein said matrix is configured to allow an aqueous solution which comprises the microorganism to flow through, thereby binding the microorganisms to said polymer on said matrix, wherein said binding is effected while maintaining viability of the microorganisms.
A method for detection and identification of viable microorganisms in an aqueous solution comprising:
- (a) contacting the solution with a water-insoluble matrix having a polymer capable of binding the microorganism covalently attached thereto via a C-terminus thereof, said polymer comprises a plurality of residues, wherein said plurality of residues comprises a plurality of amino acid residues and 3, 4, 5, 6, 7 or 8 ω-amino-fatty acid residues each being independently selected from the group consisting of 10-amino-capric acid, 12-amino-lauric acid, 14-amino-myristic acid, 16-amino-palmitic acid, 18-amino-stearic acid, 18-amino-oleic acid, 16-amino-palmitoleic acid, 18-amino-linoleic acid, 18-amino-linolenic acid and 20-amino-arachidonic acid, whereas each of said ω-amino-fatty acid residues is being covalently linked to at least two amino acid residues in said plurality of amino acid residues via an amine group of one amino acid residue and via a carboxyl of the other amino acid residue in said at least two amino acid residues, said polymer being selected from the group consisting of a linear polymer and a cyclic polymer, thereby binding the microorganisms to said polymer on said matrix, wherein said binding is effected while maintaining viability of the microorganisms;
- and
- (b) identifying the microorganism bound to said matrix.
A sterile composition comprising a water-insoluble matrix having a polymer covalently attached via a C-terminus thereof to said matrix, said polymer comprising a plurality of residues, wherein said plurality of residues comprises a plurality of amino acid residues and 3, 4, 5, 6, 7 or 8 ω-amino-fatty acid residues each being independently selected from the group consisting of 10-amino-capric acid, 12-amino-lauric acid, 14-amino-myristic acid, 16-amino-palmitic acid, 18-amino-stearic acid, 18-amino-oleic acid, 16-amino-palmitoleic acid, 18-amino-linoleic acid, 18-amino-linolenic acid and 20-amino-arachidonic acid, whereas each of said ω-amino-fatty acid residues is being covalently linked to at least two amino acid residues in said plurality of amino acid residues via an amine group of one amino acid residue and via a carboxyl of the other amino acid residue in said at least two amino acid residues, said polymer being selected from the group consisting of a linear polymer and a cyclic polymer, wherein said polymer is being capable of binding a microorganism and the composition is being identified for binding said microorganism to said polymer while maintaining viability of said microorganism.
A composition comprising a water-insoluble matrix having a polymer capable of binding a microorganism covalently attached thereto via a C-terminus thereof and further comprising microorganisms bound to said polymer, wherein said microorganisms are biologically viable, said polymer comprising a plurality of residues, wherein said plurality of residues comprises a plurality of amino acid residues and 3, 4, 5, 6, 7 or 8 ω-amino-fatty acid residues each being independently selected from the group consisting of 10-amino-capric acid, 12-amino-lauric acid, 14-amino-myristic acid, 16-amino-palmitic acid, 18-amino-stearic acid, 18-amino-oleic acid, 16-amino-palmitoleic acid, 18-amino-linoleic acid, 18-amino-linolenic acid and 20-amino-arachidonic acid, whereas each of said ω-amino-fatty acid residues is being covalently linked to at least two amino acid residues in said plurality of amino acid residues via an amine group of one amino acid residue and via a carboxyl of the other amino acid residue in said at least two amino acid residues, said polymer being selected from the group consisting of a linear polymer and a cyclic polymer.
Dependencies are inferred from the claim text.