HSP20 inhibits amyloidogenesis and neurotoxicity
AssigneeThe Texas A&M University System
InventorsAllison Ficht, Theresa Good, Ken Carson, Sundmun Lee
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Abstract
The present invention compositions and methods of using at least a portions of an isolated and purified α-crystallin polypeptide that includes one or more β-pleated sheets and that prevents neurotoxicity and amyloidogenesis.
Claims9
A method of stabilizing a protein with one or more β-pleated sheets comprising
- providing a protein in, at or about a tissue and in need of stabilization and
- contacting the protein with a heat shock polypeptide at a molar ratio of the heat shock polypeptide to the protein of between about 0.0005 and 0.001, wherein the heat shock polypeptide comprises SEQ ID NO.:
- 2 exhibiting α-crystallin activity and inhibiting the formation of Aβ multimers wherein the protein remains stable and exhibits attenuated neurotoxicity over a one-thousand-fold concentration range.
A method of stabilizing a protein with one or more β-pleated sheets comprising contacting the protein with a heat shock polypeptide wherein a molar ratio of the heat shock polypeptide to the protein is between about 0.0005 and 0.001, wherein the protein remains stable over a one-thousand-fold concentration range at 58° C. and the heat shock polypeptide comprises SEQ ID NO.:
- 2.
A method of attenuating protein aggregation comprising contacting an aggregated protein with a Hsp20, a heat shock polypeptide that comprises SEQ ID NO.:
- 2 and a molar ratio of the heat shock polypeptide to the protein of between about 0.0005 and 0.001, wherein the protein aggregation is reduced.
A method of treating a medical condition that exhibits protein aggregation with one or more β-pleated sheets comprising administering to a subject a heat shock polypeptide wherein a molar ratio of the heat shock polypeptide to the protein is between about 0.0005 and 0.001 whereby the heat shock polypeptide attenuates protein aggregation levels in a neuronal tissue of the subject and wherein the heat shock polypeptide comprises SEQ ID NO.:
- 2.
A method of attenuating protein aggregation with one or more β-pleated sheets comprising contacting an amyloidogenic protein with a heat shock polypeptide wherein a molar ratio of the heat shock polypeptide to the protein is between about 0.0005 and 0.001 and the heat shock polypeptide comprises SEQ ID NO.:
- 2.
Dependencies are inferred from the claim text.