Isolated Australian coral reef fluorescent proteins and cell-based kinase or phosphatase platforms for cancer drug development
OwnerDavid Gruber, Hung-Teh Kao +1inventors
InventorsDavid Gruber, Hung-Teh Kao, Vincent Pieribone
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Abstract
The present invention concerns novel isolated fluorescent proteins, variants thereof, and polynucleotides encoding the same. Methods for making and using the polypeptides and polynucleotides are also provided. For example, methods to detect protein-protein interactions, to develop novel fluorescent reagents, to monitor cellular events, as well as cell-based methods for screening for kinase or phosphatase inhibitors, are set forth. Kits to carry out the methods of the invention are also taught.
Claims9
An isolated fluorescent polypeptide comprising an amino acid sequence selected from the group consisting of SEQ ID NOS:
- 10, 12, 14, 16, 18, 20, 22, 24, 26, 28, 30, 32, 34, 36, 38, 40, 42 and 44.
An isolated fluorescent polypeptide variant, wherein the fluorescent polypeptide variant comprises at least 90% sequence identity to SEQ ID NO:
- 10, 12, 14, 16, 18, 20, 22, 24, 26, 28, 30, 32, 34, 36, 38, or 40.
A fusion protein comprising an amino acid sequence of a protein of interest operatively joined to an amino acid sequence selected from the group consisting of SEQ ID NOS:
- 10, 12, 14, 16, 18, 20, 22, 24, 26, 28, 30, 32, 34, 36, 38, 40, 42 and 44.
A fusion protein comprising an amino acid sequence of a protein of interest operatively joined to an amino acid sequence having at least 90% sequence identity to SEQ ID NO:
- 10, 12, 14, 16, 18, 20, 22, 24, 26, 28, 30, 32, 34, 36, 38, or 40.
An isolated protein comprising the amino acid sequence of SEQ ID NO:
- 10 and further comprising at least one heterologous consensus sequence for phosphorylation by a protein kinase inserted into the amino acid sequence of SEQ ID NO:
- 10.
Dependencies are inferred from the claim text.