Back to results
US 8,709,823 B2Granted
Single-wavelength correction method for luminescent homogeneous biological assay
AssigneeBiophor Diagnostics, Inc.
InventorTodd M. Corneillie
Life of this patent
Estimating the term…
Abstract
A method for performing time resolved homogeneous assays using a long-lifetime luminescent dye as a donor. A reaction well containing a sample portion, donor reagent, and acceptor reagent and a matrix well containing a sample portion and donor reagent are excited and the resulting emission from each is measured at a single wavelength associated with the acceptor. The measurement obtained from the matrix well is used to provide a correction for the measurement obtained from the reaction well. The sample may be a biological fluid such as an oral fluid.
Claims20
A method for performing a homogeneous biological assay comprising:
- preparing at least one reaction volume and a matrix volume wherein said reaction volume and said matrix volume each comprise a biological fluid;
- adding an acceptor reagent to said reaction volume;
- adding a lanthanide donor reagent to said reaction volume;
- adding a lanthanide donor reagent to said matrix volume;
- applying an optical excitation to said reaction volume;
- obtaining a first integrated intensity value for the luminescent intensity of said reaction volume at a single characteristic emission wavelength of said acceptor reagent;
- applying an optical excitation to said matrix volume;
- obtaining a second integrated intensity value for the luminescent intensity of said matrix volume at said single characteristic emission wavelength of said acceptor reagent, wherein said luminescent intensity of said matrix volume comprises luminescent emission from said lanthanide donor reagent at said single characteristic emission wavelength of said acceptor reagent;
- and
- correcting said first integrated intensity value by applying a mathematical expression to said first integrated intensity wherein said mathematical expression comprises said second integrated intensity value.
7 more elements
Dependencies are inferred from the claim text.