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US 8,709,806 B2Granted
EpCAM as a reprogramming factor for non-pluripotent cells
AssigneeAcademia Sinica
InventorsHan-Chung Wu, Tung-Ying Lu, Cheng-Fu Kao, John Yu, Ruei-Min Lu, Mei-Ying Liao, Hung-Chih Kuo
Life of this patent
Estimating the term…
Abstract
Methods are provided for inducing non-pluripotent cells to become pluripotent. Methods also include identifying and isolating induced pluripotent (iPS) cells and uses thereof. Compositions and kits for carrying out the subject methods are also provided.
Claims5
A method for producing mammalian induced pluripotent stem cells (iPS cells) the method comprising:
- introducing retroviral vectors comprising a nucleic acid encoding Oct4, Sox2, Klf4, cMyc and epithelial cell adhesion molecule (EpCAM) into an isolated mammalian non-pluripotent cell,
- culturing the cell under conditions that reprogram the non-pluripotent cells into an iPS cell that exhibits self-renewal and pluripotency.
Dependencies are inferred from the claim text.