Device for preparing a body fluid for a bacteriological analysis

A device for preparation of a fluid sample for analysis, comprising:

• a container comprising an upper part and a lower part that configured to be connected to each other to together form a chamber, said lower part comprising a base, wherein the upper part comprises a stopper configured to allow air intake into the upper volume, when unscrewed;
• a piston that extends from the base of the lower part into the chamber, said piston comprising a closure means, said piston being movable between an open position and closed position, wherein when the piston is in a closed position, the closure means of the piston separates the chamber into an upper volume and a lower volume, and wherein when the piston is in an open position, the upper volume and lower volume are in fluid communication;
• a separation zone that defines a bottleneck, wherein the lower volume extends under the bottleneck, wherein the closure means cooperates with the separation zone in order to define the upper volume and lower volume;
• and
• a means for introduction of a the fluid into the upper volume of the chamber.