A method of manufacturing a column reactor capable of producing nitrate nitrogen as an inorganic nutrient from an organic material, the method comprising:
- (i) integrally molding a solid support (A) having gas permeability so as to maintain a solid shape, said solid support (A) comprising one or more porous carriers selected from the group consisting of rockwool, vermiculite, pearlite, zeolite, sand, glass, ceramic, urethane, nylon, a melamine resin, cedar chips, bog moss, filter paper and agar;
- (ii) adding thereto microorganisms capable of conducting a multiple parallel mineralization by mineralization of an organic material to produce nitrate nitrogen;
- (iii) subsequently adding an organic material (B) in an amount of 0.01 to 20 g, in terms of dry weight, with respect to 1 L of the carrier and then adding water, said organic material (B) comprising one or more organic materials selected from the group consisting of a fish-based soluble fertilizer, fish flour, an oil cake, raw garbage, corn steep liquor, rice bran, soybean flour, a plant residue, milk, powdered milk and livestock manure;
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- (iv) then leaving the resultant material at rest until nitrate nitrogen starts to be produced in an effluent during washing of the carrier by an addition of water to discharge the effluent from the carrier;
- thereby obtaining a carrier on which the microorganisms capable of conducting a multiple parallel mineralization are immobilized.