Targeted gene deletions for polysaccharide slime formers
AssigneeCP Kelco U.S., Inc.
InventorsNancy E. Harding, Yamini N. Patel, Russell J. Coleman
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Abstract
The present invention provides improved polysaccharides (e.g., gellan and diutan) produced by mutant gene M or gene N Sphingomonas strains containing at least one genetic modification that favors slime-forming polysaccaride production. Methods of making the mutant Sphingomonas strains and the culture broth containing such mutant Sphingomonas strains are also provided.
Claims11
An isolated slime forming mutant Sphingomonas strain comprising a substitution, insertion or deletion in gene dpsN in a diutan biosynthetic gene cluster that inactivates gene dpsN and favors the production of a slime form polysaccharide, wherein said dpsN comprises the nucleotide sequence of nucleotides 1321-2019 as set forth in SEQ ID NO:14.
Dependencies are inferred from the claim text.