Method for producing purified influenza virus antigen
AssigneeDenka Seiken Co., Ltd.
InventorsKoichi Takahashi, Kazuaki Maeda, Noriyuki Izutani, Shinichi Yanagi, Yukiko Nakashima
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Abstract
The present invention aims to provide a method which enables efficient removal of impurities such as the host proteins from an influenza virus culture liquid by a simple operation, allowing separation and purification of an influenza virus antigen. The method of the present invention for producing a purified influenza virus antigen comprises the step of treating a sample containing an influenza virus with a surfactant, the step of bringing the sample after the treatment into contact with hydroxyapatite in the presence of the surfactant, and the step of recovering a hydroxyapatite-non-adsorbed fraction.
Claims17
A method for producing a purified influenza virus hemagglutinin (HA) antigen, said method comprising the steps of:
- treating a sample containing the influenza virus HA antigen with a surfactant;
- bringing said sample after the treatment into contact with hydroxyapatite in the presence of said surfactant under conditions where most of the HA antigen is not adsorbed by hydroxyapatite;
- and
- recovering a fraction not adsorbed by the hydroxyapatite,
- wherein said surfactant comprises at least one of an amphoteric surfactant 3-(3-cholamidepropyl)dimethylammonio-1-propanesulphonate, an nonionic surfactant polyoxyethyeneglycol p-t-octylphenyl ether and an anioni surfactant deoxycholic acid or a salt thereof.
Dependencies are inferred from the claim text.