Sperm factor sequences
AssigneeUniversity College Cardiff Consultants Limited
InventorFrancis Anthony Lai
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Abstract
A nucleic acid sequence, including an isolated, purified or recombinant nucleic acid sequence, includes: (a) a nucleic acid sequence encoding a polypeptide encompassed by the present invention, namely, a PLC-zeta; PLCζ amino acid sequence, capable of triggering calcium oscillations in oocytes; (b) a sequence substantially homologous to or that hybridizes to sequence (a) under stringent conditions; (c) a sequence substantially homologous to or that hybridizes to the sequences (a) or (b) but for degeneracy of the genetic code; and (d) an oligonucleotide specific for any of the sequences (a), (b) or (c) above.
Claims3
A diagnostic method for determining the fertility status of a mammal, comprising determining the amount of a nucleic acid as set forth in SEQ ID NO. 3 or a sequence which hybridizes thereto under stringent conditions, present or absent in a test sample comprising a sperm cell obtained from the mammal;
- wherein said stringent conditions comprise incubation at from about 42° C. to about 68° C. in a solution comprising 6×SSC or 6×SSPE and one or more washing steps at 65° C.;
- and
- further wherein the amount of nucleic acid in the test sample is indicative of expression of a phospholipase C (PLC) ζ per sperm cell of less than 170 femtograms or more than 410 femtograms, which amount is indicative that the sperm cell will be unable to stimulate an oocyte to develop to a blastocyst stage and so that the mammal is at risk of infertility.
Dependencies are inferred from the claim text.