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AssigneeColorado School of Mines
InventorsRandor Radakovits, Robert Jinkerson, Matthew Posewitz
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The present disclosure is directed to novel polynucleotide sequences for use in Nannochloropsis gaditana. The novel polynucleotide sequences include control sequences and coding sequences. Also disclosed are novel gene expression constructs wherein N. gaditana promoters/control regions are operatively linked to N. gaditana or non-N. gaditana coding sequences. These novel polynucleotide sequences and expression constructs can be introduced into N. gaditana and can recombine into the N. gaditana genome. Expression from these polynucleotide sequences and expression constructs can enhance N. gaditana biomass and/or lipid biosynthesis. Also disclosed are methods for modifying N. gaditana, for example by stably transforming N. gaditana with nucleic acid sequences, growing the modified N. gaditana, and obtaining biomass and biofuels from the modified N. gaditana.
A transgenic algae of the genus Nannochloropsis transformed with a purified polynucleotide sequence comprising the Nannochloropsis control sequence of SEQ ID NO:7604 operably linked to a polypeptide coding sequence, said purified polynucleotide sequence further comprising a selectable marker.
A method of making a transgenic algae comprising:
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