Back to results
US 8,709,765 B2Granted
Manipulation of an alternative respiratory pathway in photo-autotrophs
AssigneeAurora Algae, Inc.
InventorsShaun Bailey, Bertrand Vick, Jeff Moseley
Life of this patent
Estimating the term…
Loading patent…
Loading patent…
AssigneeAurora Algae, Inc.
InventorsShaun Bailey, Bertrand Vick, Jeff Moseley
Estimating the term…
Exemplary methods for increasing TAG production in an algal cell during imbalanced growth conditions are provided. Some methods comprise knocking out an AOX gene, wherein the AOX gene produces an amino acid sequence having substantial similarity to the amino acid sequence of SEQ. ID. NO. 2. In further methods, the algal cell may be of genus Nannochloropsis. The AOX gene may be replaced by a construct having a nucleotide sequence having substantial similarity to SEQ ID. NOS. 3 through 5 (inclusive), wherein each of the sequences are next to or in close proximity to one another in a linear fashion. In some methods, the AOX gene may be replaced via homologous recombination. As a result, lipid production by the selected recombinant algal cell may be increased over that produced by a wild-type algal cell.
A method for increasing triacylglyceride (“TAG”) production in an algal cell during imbalanced growth conditions, the method comprising:
The method of claim 3, wherein the AOX gene is replaced by the construct of claim 4 via homologous recombination.
A transformation method for introducing deoxyribonucleic acid (DNA) into a nucleus of an algal cell, the method comprising:
Dependencies are inferred from the claim text.