Polypeptides and immunizing compositions containing gram positive polypeptides and methods of use
AssigneeEpitopix, LLC
InventorsDaryll A. Emery, Darren E. Straub, Laura Wonderling, Lisa L. Herron Olson
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Abstract
The present invention provides isolated polypeptides isolatable from a Staphylococcus spp. Also provided by the present invention are compostions that include one or more of the polypeptides, and methods for making and methods for using the polypeptides.
Claims33
A method of isolating a metal-regulated polypeptide from Staphylococcus cells, the method comprising:
- growing the Staphylococcus cells in a medium that comprises 2,2′-dipyridyl at a concentration of at least about 800 μM under conditions effective for the cell to express at least one metal-regulated polypeptide;
- and
- isolating the at least one metal-regulated polypeptide from the cells.
A method of isolating a metal-regulated polypeptide from cells, the method comprising:
- growing a seed stock of cells;
- transferring at least a portion of the seed stock to a culture comprising a chelator at a concentration of at least about 300 μg/mL, thereby forming a working seed;
- growing the working seed under conditions effective for the cells to express at least one metal regulated polypeptide;
- and
- isolating the metal-regulated polypeptide from cells of the working seed.
A method of preparing antibody that specifically binds to a metal-regulated polypeptide natively expressed by Staphylococcus cells, the method comprising:
- growing the Staphylococcus cells that express the metal-regulated polypeptide in a medium that comprises 2,2′-dipyridyl at a concentration of at least about 800 μM under conditions effective for the cell to express the metal-regulated polypeptide;
- isolating the metal-regulated polypeptide from the cells;
- and
- preparing antibody that specifically binds the isolated metal-regulated polypeptide.
Dependencies are inferred from the claim text.