Control of protein activity using a conducting polymer
AssigneeThe United States of America as represented by the Secretary of the Navy
InventorsBrett D. Martin, Luminita M. Velea, Banahalli Ratna, Bruce P. Gaber
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Abstract
A device having a substrate and an enzyme attached to the substrate. The substrate has a polymeric surface having at least two conductivity states. A minimum voltage that does not cause a redox reaction in the enzyme may be applied to the polymeric surface to change the conductivity state of the surface. A method of controlling enzyme activity by providing the above substrate with polymeric surface, attaching an enzyme to the substrate, and altering the conductivity state of the polymeric surface. Changing the conductivity of the polymer can change the activity of the enzyme.
Claims11
A device comprising:
- a substrate comprising poly(2-hydroxymethyl thieno[3,4-b]-1,4-dioxane);
- a streptavidin non-covalently adsorbed onto the poly(2-hydroxymethyl thieno[3,4-b]-1,4-dioxane);
- and
- kinesin-biotin carboxyl carrier protein non-covalently bound to the streptavidin.
A method of controlling enzyme activity comprising:
- providing a substrate comprising poly(2-hydroxymethyl thieno[3,4-b]-1,4-dioxane);
- non-covalently adsorbing streptavidin onto the poly(2-hydroxymethyl thieno[3,4-b]-1,4-dioxane);
- non-covalently binding kinesin-biotin carboxyl carrier protein to the streptavidin;
- and
- applying a voltage to the poly(2-hydroxymethyl thieno[3,4-b]-1,4-dioxane) that changes the poly(2-hydroxymethyl thieno[3,4-b]-1,4-dioxane) from a doped form to a dedoped form.
Dependencies are inferred from the claim text.