Bitter taste receptors
AssigneeDeutsches Institut für Ernährungsforschung, Stiftung des öffentlichen Rechts, Vertreten durch den Stiftungsvorstand
InventorsBernd Bufe, Thomas Hofmann, Dietmar Krautwurst, Christina Kuhn, Wolfgang Meyerhof
Life of this patent
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Abstract
The present invention relates to bitter-taste receptors and their role in bitter taste transduction. The invention also relates to assays for screening molecules that modulate, e.g. suppress or block, bitter taste transduction, or enhance bitter taste response.
Claims4
A process for identifying a compound that binds to a polypeptide selected from the group consisting of:
- (a) a polypeptide comprising the amino acid sequence as shown in SEQ ID NO:37;
- (b) a fragment or derivative of a polypeptide of (a), wherein in said derivative no more than 15 amino acid residues are conservatively substituted compared to said polypeptide, wherein said fragment comprises at least 290 contiguous amino acids of SEQ ID NO:37 and said fragment or derivative has bitter substance binding activity;
- and
- (c) a polypeptide which is at least 95% identical to a polypeptide as defined in any one of (a) to (b) and which has bitter substance binding activity;
- and
- wherein said process comprises:
- (1) contacting said polypeptide, or a host cell genetically engineered with a polynucleotide encoding said polypeptide, or with a vector containing a polynucleotide encoding said polypeptide, with a compound;
- (2) detecting the presence of the compound which binds to said polypeptide;
- and
- (3) determining whether the compound binds said polypeptide;
- wherein prior to, concomitantly and/or after step (1) said polypeptide or said host cell is contacted with an agonist selected from the group consisting of strychnine, brucine, and functional derivatives thereof.
A process for identifying an antagonist of the bitter taste receptor activity of the polypeptide selected from the group consisting of:
- (a) a polypeptide comprising the amino acid sequence as shown in SEQ ID NO:37;
- (b) a fragment or derivative of a polypeptide of (a), wherein in said derivative no more than 15 amino acid residues are conservatively substituted compared to said polypeptide, wherein said fragment comprises at least 290 contiguous amino acids of SEQ ID NO:37 and said fragment or derivative has bitter taste receptor activity;
- and
- (c) a polypeptide which is at least 95% identical to a polypeptide as defined in any one of (a) to (b) and which has bitter taste receptor activity;
- and
- wherein said process comprises:
- (1) contacting said polypeptide, or a host cell genetically engineered with a polynucleotide encoding said polypeptide or with a vector containing a polynucleotide encoding said polypeptide, with a potential antagonist;
- and
- (2) determining whether the potential antagonist antagonizes the bitter taste receptor activity of said polypeptide;
- wherein prior to, concomitantly and/or after step (1) said bitter taste receptor or said host cell is contacted with an agonist selected from the group consisting of strychnine, brucine, and functional derivatives thereof.
Dependencies are inferred from the claim text.