Method of making an array of nucleic acid colonies
AssigneeIllumina, Inc.
InventorsMostafa Ronaghi, Helmy Eltoukhy
Life of this patent
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Abstract
A method of making an array of nucleic acid colonies, including the steps of (a) providing a substrate having a patterned surface of features, wherein the features are spatially organized in a repeating pattern on the surface of the substrate; (b) contacting the substrate with a solution of different target nucleic acids to seed no more than a subset of the features that contact the solution; (c) amplifying the nucleic acids on the subset of features; and (d) repeating steps (b) and (c) to increase the number of features that are seeded with a nucleic acid, thereby making an array of nucleic acid colonies.
Claims20
A method of making an array of nucleic acid colonies, comprising
- (a) providing a substrate comprising a patterned surface of features, wherein the features are spatially organized in a repeating pattern on the surface of the substrate;
- (b) contacting the substrate with a solution of different target nucleic acids to seed no more than a subset of the features that contact the solution;
- (c) amplifying the nucleic acids on the subset of features;
- and
- (d) repeating steps (b) and (c) to increase the number of features that are seeded with a nucleic acid, thereby making an array of nucleic acid colonies.
Dependencies are inferred from the claim text.