Compositions substantially free of sodium chloride and methods for the storage of red blood cells
OwnerJohn R. Hess, Tibor J. Greenwaltinventors
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Abstract
The present invention provides an aqueous composition for storage of red blood cells consisting essentially of: adenine; dextrose; at least one nonmetabolizable membrane-protectant sugar; and a specifically defined pH buffering system. Also provided are improved methods for preserving red blood cells and methods for increasing the viability, membrane retention, and recoverability while suppressing apoptosis, hemolysis, and post-reinfusion clearance of stored red blood cells which utilize the novel compositions.
Claims17
A composition for the storage of red blood cells (RBCs), said composition substantially free of sodium chloride and consisting essentially of:
- about 2 mM adenine,
- about 80 mM dextrose,
- about 55 mM mannitol,
- about 26 mM sodium bicarbonate, and
- about 12 mM disodium phosphate.
A method of preserving red blood cells (RBCs) for a storage period comprising:
- (a) mixing an amount of collected whole blood containing the RBCs to be stored and plasma with an anticoagulant solution, thereby forming a suspension of collected whole blood;
- (b) treating the suspension of collected whole blood to deplete the plasma and concentrate the RBCs, thereby forming packed RBCs;
- (c) mixing the packed RBCs with an amount of a composition, wherein the ratio of the amount of composition to the amount of whole blood collected is about 1:4.5 and wherein the composition is substantially free of sodium chloride and consists essentially of about 2 mM adenine, about 80 mM dextrose, about 55 mM mannitol, about 26 mM sodium bicarbonate, and about 12 mM disodium phosphate;
- (d) cooling the suspension of RBCs to about 1 to about 6° C.;
- and
- (e) storing the cooled suspension of RBCs for the storage period.
A method of preserving red blood cells (RBCs) for a storage period comprising:
- (a) mixing a sample of collected whole blood containing the RBCs to be stored and plasma with an anticoagulant solution, thereby forming a suspension of collected whole blood;
- (b) treating the suspension of collected whole blood to deplete the plasma and concentrate the RBCs, thereby forming packed RBCs;
- (c) mixing the packed RBCs with an amount of a composition sufficient to form a suspension of RBCs having about 35% to about 70% RBCs by volume, wherein the composition is substantially free of sodium chloride and consists essentially of about 2 mM adenine, about 80 mM dextrose, about 55 mM mannitol, about 26 mM sodium bicarbonate, and about 12 mM disodium phosphate;
- (d) cooling the suspension of RBCs to about 1 to about 6° C.;
- and
- (e) storing the cooled suspension of RBCs for the storage period.
A method of preserving red blood cells (RBCs) for a storage period comprising:
- (a) mixing packed RBCs with an amount of a composition sufficient to form a suspension of RBCs having about 35% to about 70% RBCs by volume, and wherein the composition is substantially free of sodium chloride and consists essentially of about 2 mM adenine, about 80 mM dextrose, about 55 mM mannitol, about 26 mM sodium bicarbonate, and about 12 mM disodium phosphate;
- (b) cooling the suspension of RBCs to about 1 to about 6° C.;
- and
- (c) storing the cooled suspension of RBCs for the storage period.
Dependencies are inferred from the claim text.