Closed cell culture system
AssigneeNext21 K.K.
InventorShigeki Suzuki
Life of this patent
Estimating the term…
Abstract
An object of the present invention is to provide a tissue cell culture system whereby a call tissue can be efficiently and quickly proliferated in vivo and the onset bacterial infection in an injured part can be avoided in the course of a treatment. More specifically, a closed cell culture system (1) characterized in that a defection (2) of a tissue on the body surface or inside the body is tightly sealed to form a closed environment free from the invasion of bacteria, etc. and then a solution appropriate for cell culture is circulated in the tissue defection thus sealed to thereby regenerate the defective tissue; and a method of administering a drug which comprises dissolving a remedy in the perfusion with the use of the above system and thus promoting the treatment of the defection.
Claims8
A closed cell culture system, comprising:
- a) a bioabsorbable material configured to be embedded within a tissue defect inside a body of a patient to form a cell proliferation site and to be a foundation on which regenerating tissue cells can adhere and proliferate as the tissue defect heals and closes up;
- b) a perfusion introduction tube configured to provide a solution to the bioabsorbable material;
- c) a perfusion discharge tube configured to discharge the solution;
- d) a circuit for circulating the solution, the circuit perfusing the solution within the tissue defect by way of an entrance portion of the perfusion introduction tube, the circuit discharging the solution from the tissue defect by way of an exit portion of the perfusion discharge tube, the solution being appropriate for cell culture;
- and
- e) a puncture needle and a balloon catheter, the balloon catheter passing through and within the puncture needle and being inflated to create the tissue defect,
- wherein the closed cell culture system is capable of regenerating tissue at the cell proliferation site of the tissue defect.
A method of regenerating tissue, comprising:
- creating a tissue defect within a human body, the tissue defect being created by a puncture needle and a balloon catheter, the puncture needle being inserted into a human body, the balloon catheter passing through and within the puncture needle and being inflated to create the tissue defect, the tissue defect being a cell proliferation site;
- depositing a bioabsorbable material within the tissue defect through the puncture needle;
- removing the puncture needle from the body;
- setting a perfusion introduction tube to provide a solution to the bioabsorbable material, the solution being appropriate for cell culture;
- setting a perfusion discharge tube to discharge the solution;
- perfusing the solution within the tissue defect by way of an entrance portion of the perfusion introduction tube;
- and
- discharging the solution from the tissue defect by way of an exit portion of the perfusion discharge tube.
Dependencies are inferred from the claim text.