Engineered enzymes with methionine-gamma-lyase enzymes and pharmacological preparations thereof
AssigneeAEMase, Inc.
InventorsGeorge Georgiou, Everett Stone
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Abstract
Methods and composition related to the engineering of a novel protein with methionine-γ-lyase enzyme activity are described. For example, in certain aspects there may be disclosed a modified cystathionine-γ-lyase (CGL) comprising one or more amino acid substitutions and capable of degrading methionine. Furthermore, certain aspects of the invention provide compositions and methods for the treatment of cancer with methionine depletion using the disclosed proteins or nucleic acids.
Claims19
A polypeptide comprising a variant of a primate cystathionine gamma-lyase, wherein the variant crysathionine gamma lyase has methionine gamma-lyase activity, a sequence at least 95% identical to SEQ ID NO:1, and comprises amino acid substitutions at amino acid positions corresponding to positions 59, 119 and/or 339 of SEQ ID NO:
- 1, the native human cystathionine gamma lyase, said substitutions being i) E59V or E59N, ii) R119L and iii) E339V.
Dependencies are inferred from the claim text.