Methods and compositions for wound healing

A method of treatment comprising:

• a) providing a subject having a wound comprising a wound bed presenting amine and sulfhydryl groups, at least one bifunctional covalent modification agent that covalently reacts with a chemical entity selected from the group consisting of amine and sulfhydryl groups, and at least one wound active agent;
• b) contacting said wound with said at least one bifunctional covalent modification agent that covalently reacts with a chemical entity selected from the group consisting of amine and sulfhydryl groups in said wound bed to provide a covalently modified wound bed, and
• c) contacting said covalently modified wound bed with said at least one wound active agent under conditions such that said at least one wound active agent is incorporated into said modified wound bed by covalently binding to said bifunctional covalent modification agent and healing of said wound is enhanced, wherein said wound active agent is selected from the group consisting of trophic factors, enzymes, enzyme inhibitors, defensins, anti-infective agents, analgesics, anticoagulants, anti-inflammatory agents, vasoconstrictors, vasodilators, diuretics, and anti-cancer agents.