Cellular scaffold
AssigneeStemedica Cell Technologies, Inc.
InventorsAlexander Kharazi, Ludmilla Kharazi, Nikolai Tankovich
Life of this patent
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Abstract
A cellular scaffold that is suitable for tissue regeneration, cell culture and in vitro assays. The invention relates to a layered cell scaffold that is seeded with mesenchymal and ectodermal cells. The layered cellular scaffold comprises an inoculum of mesenchymal cells and ectodermal cells positioned between two opposing scaffolds in a sandwich configuration. The layered cell scaffold provides a functional skin equivalent that is suitable for transplantation and in vitro cell-based assays.
Claims20
A cellular scaffold arrangement comprising:
- a first collagen cell scaffold having a first surface and a second surface wherein said second surface of said first cell scaffold is seeded with mesenchymal
- a second collagen cell scaffold having a first surface and a second surface wherein said first surface of said second cell scaffold is seeded with ectodermal cells;
- and
- a composition comprising cell -factors in contact with at least one of said first collagen cell scaffold, said second collagen cell scaffold, said mesenchymal cells and said ectodermal cells;
- wherein said second surface of said first scaffold is in contact with said first surface of said second scaffold;
- wherein said second cell scaffold has the consistency of a sponge.
A cellular scaffold arrangement comprising:
- a first collagen cell scaffold having a first surface and a second surface wherein said second surface of said first cell scaffold is seeded with mesenchymal cells;
- a second collagen cell scaffold having a first surface and a second surface wherein said first surface of said second cell scaffold is seeded with ectodermal cells;
- and
- neural stem cells in contact with at least one of said first collagen cell scaffold, said second collagen cell scaffold, said mesenchymal cells and said ectodermal cells;
- wherein said second surface of said first scaffold is in contact with said first surface of said second scaffold;
- wherein said second cell scaffold has the consistency of a sponge.
Dependencies are inferred from the claim text.